Overview

  • Product name

    Anti-Biotin antibody (HRP)
    See all Biotin primary antibodies
  • Description

    Rabbit polyclonal to Biotin (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Specificity

    Reacts with free biotin and biotinylated antibody and proteins.
  • Tested applications

    Suitable for: WB, Competitive ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to Biotin conjugated to keyhole limpet haemocyanin.

  • General notes

    Reductive amination; Ab/HRP ratio=1/10.

Properties

Applications

Our Abpromise guarantee covers the use of ab53468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.25 - 1 µg/ml.
Competitive ELISA Use a concentration of 0.25 - 1 µg/ml.

Target

  • Relevance

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names

    • Vitamin B4 antibody

References

This product has been referenced in:

  • Króliczewski J  et al. ALB3 Insertase Mediates Cytochrome b6 Co-translational Import into the Thylakoid Membrane. Sci Rep 6:34557 (2016). WB . Read more (PubMed: 27698412) »
See 1 Publication for this product

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