Anti-Biotin antibody [Hyb-8] (ab201341)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [Hyb-8] (ab201341)](https://www.abcam.com/ps/products/201/ab201341/Images/ab201341-243025-anti-biotin-antibody-hyb-8-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [Hyb-8] (ab201341)")
Key features and details
- Mouse monoclonal [Hyb-8] to Biotin
- Suitable for: Flow Cyt, In situ hybridization, WB, ICC/IF, IHC-P
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-Biotin antibody [Hyb-8]
See all Biotin primary antibodies -
Description
Mouse monoclonal [Hyb-8] to Biotin -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, In situ hybridization, WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Other Immunogen Type corresponding to Biotin. (Biotinylated sheep immunoglobulin).
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Positive control
- Biotinylated proteins in solution or on tissues.
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General notes
ab201341 has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
ab201341 is purified from Bioreactor Concentrate by Protein A/G column chromatography. -
Clonality
Monoclonal -
Clone number
Hyb-8 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab201341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.5-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| In situ hybridization | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 0.5 - 1 µg/ml. | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues. |
Target
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Relevance
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
Alternative names
- Vitamin B4 antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Biotin antibody [Hyb-8] (ab201341)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue stained with biotinylated Lambda Light Chain probe followed by ab201341 at 1 µg/ml.
Protocols
References (3)
ab201341 has been referenced in 3 publications.
- Shaw A et al. Binding to nanopatterned antigens is dominated by the spatial tolerance of antibodies. Nat Nanotechnol 14:184-190 (2019). PubMed: 30643273
- Powell ML et al. New Fpg probe chemistry for direct detection of recombinase polymerase amplification on lateral flow strips. Anal Biochem 543:108-115 (2018). PubMed: 29233680
- Roussis IM et al. RNA Whole-Mount In situ Hybridisation Proximity Ligation Assay (rISH-PLA), an Assay for Detecting RNA-Protein Complexes in Intact Cells. PLoS One 11:e0147967 (2016). PubMed: 26824753
