Overview

  • Product name

    Anti-Biotin antibody [Hyb-8] - BSA and Azide free
    See all Biotin primary antibodies
  • Description

    Mouse monoclonal [Hyb-8] to Biotin - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    ab234026 is highly specific to biotin and shows no cross-reaction with other structurally related compounds.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Other Immunogen Type corresponding to Biotin. Biotinylated sheep immunoglobulin

  • Positive control

    • IHC-P: Human tonsil tissue.
  • General notes

    ab234026 has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Properties

Applications

Our Abpromise guarantee covers the use of ab234026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml.

Incubate with primary antibody for 30 minutes at room temperature.

Target

  • Relevance

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names

    • Vitamin B4 antibody

Images

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Biotin using ab234026 at 1 μg/ml in immunohistochemical analysis.

References

ab234026 has not yet been referenced specifically in any publications.

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