Biotin Anti-Apolipoprotein CIII antibody (ab21024)
Key features and details
- Biotin Goat polyclonal to Apolipoprotein CIII
- Suitable for: WB, ELISA
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Apolipoprotein CIII antibody
See all Apolipoprotein CIII primary antibodies -
Description
Biotin Goat polyclonal to Apolipoprotein CIII -
Host species
Goat -
Conjugation
Biotin -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Purified human Apolipoprotein CIII.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.0268% PBS, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Human Apo CIII-Sepharose affinity column and labeled with Biotin. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ELISA pair antibody
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab21024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/8000 - 1/30000. Predicted molecular weight: 11 kDa. Dilution optimised using Chromogenic detection. | |
| ELISA | 1/8000 - 1/30000. Can be paired for ELISA with Rabbit polyclonal to Apolipoprotein CIII (ab21032). |
Target
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Function
Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. -
Tissue specificity
Constitutes 50% of the protein fraction of VLDL and 2% of that of HDL. Synthesized predominantly in liver and to a lesser degree in intestine. -
Involvement in disease
Defects in APOC3 may be a cause of hyperalphalipoproteinemia (HYPALIP) [MIM:143470]. Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). -
Sequence similarities
Belongs to the apolipoprotein C3 family. -
Post-translational
modificationsO-linked glycan consists of Gal-GalNAc disaccharide, further modified with up to 3 sialic acid residues. O-glycosylated on Thr-94 with a core 1 or possibly core 8 glycan. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 345 Human
- Omim: 107720 Human
- SwissProt: P02656 Human
- Unigene: 73849 Human
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Alternative names
- APOC3 antibody
- APO C3 antibody
- Apo CIII antibody
see all
Datasheets and documents
References (3)
ab21024 has been referenced in 3 publications.
- Khetarpal SA et al. A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels. Nat Med 23:1086-1094 (2017). PubMed: 28825717
- Wang Y et al. Development of a sensitive ELISA to quantify apolipoprotein CIII in nonhuman primate serum. J Lipid Res 52:1265-71 (2011). Sandwich ELISA ; Cynomolgus monkey . PubMed: 21371998
- Dernick G et al. Multidimensional profiling of plasma lipoproteins by size exclusion chromatography followed by reverse-phase protein arrays. J Lipid Res 52:2323-31 (2011). PubMed: 21971713
