Biotin Anti-Asparaginase antibody (ab34616)
Key features and details
- Biotin Rabbit polyclonal to Asparaginase
- Suitable for: Immunomicroscopy, WB, ELISA
- Reacts with: Escherichia coli
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Asparaginase antibody
See all Asparaginase primary antibodies -
Description
Biotin Rabbit polyclonal to Asparaginase -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: Immunomicroscopy, WB, ELISAmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Asparaginase (Escherichia coli )
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
This antibody was purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab34616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Immunomicroscopy | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. Predicted molecular weight: 37 kDa. | |
| ELISA | Use at an assay dependent concentration. |
Target
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Relevance
Asparaginase is an enzyme purified from E. coli and Erwinia carotovora. It acts by deaminating extracellular L asparagine, an amino acid that appears to be essential for protein synthesis by some tumour cells which are unable to synthesise asparagine. Asparaginase from Erwinia carotovora is serologically and biochemically distinct from asparaginase from E. coli, although its antineoplastic activity and toxicity is similar. Asparaginase is usually considered to be cell cycle phase nonspecific, but it may block some cells in G1 or S phase. Asparaginase reduces cellular and humoral immunity. E.coli contains two L asparaginase isoenzymes: L asparaginase I, a low affinity enzyme located in the cytoplasm, and L asparaginase II, a high affinity secreted enzyme. -
Cellular localization
Cytoplasmic, Secreted and Periplasm. -
Database links
- Entrez Gene: 1036607 Escherichia coli
- Entrez Gene: 1039460 Escherichia coli
- Entrez Gene: 946278 Escherichia coli
- Entrez Gene: 959650 Escherichia coli
- SwissProt: P00805 Escherichia coli
- SwissProt: P0A962 Escherichia coli
- SwissProt: P0A963 Escherichia coli
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Alternative names
- AnsA antibody
- AnsB antibody
- Colaspase antibody
see all
Images
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Western blot - Biotin Anti-Asparaginase antibody (ab34616)Biotin Anti-Asparaginase antibody (ab34616) at 1/1000 dilution + L-asparaginase at 0.1 µg
Secondary
HRP-Streptavidin at 1/40000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Protocols
References (0)
ab34616 has not yet been referenced specifically in any publications.
