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    biotin-assay-kit-colorimetric-ab185441.pdf

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Biotin Assay Kit (Colorimetric) (ab185441)

  • Datasheet
  • SDS
  • Protocol Booklet
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Biotin Standard Curve
  • Amount of biotin vs amount of biotinylated BSA

Key features and details

  • Assay type: Semi-quantitative
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Sample type: Purified protein
  • Sensitivity: 15 ng

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Overview

  • Product name

    Biotin Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Purified protein
  • Assay type

    Semi-quantitative
  • Sensitivity

    15 ng
  • Product overview

    Abcam’s Biotin Quantitation Kit (Colorimetric) (ab185441) is based on the differential binding of Streptavidin to Biotin and a dye, 2-(4-hydroxyazobenzene) benzoic acid (HABA). When HABA is bound to Streptavidin, addition of free biotin or biotinylated biomolecules results in its displacement in the reaction. These changes are manifested in terms of decrease in the overall absorption (OD 500 nm) and can be quantitatively correlated to the amount of biotin present in solution. The kit also provides Biotin as a Standard and Biotinylated BSA as a positive control. This kit can be used to estimate as low as 60 pmol or 15 ng of biotin in solution.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K811 Biotin Quantitation Kit (Colorimetric). K811-100 is the same size as the 100 test size of ab185441.

    Biotin (Vitamin H) is an important biomolecule that has been widely used in Biotinylation reactions to label antibodies or other proteins of interest. Due to its low molecular weight, biotin usually does not cause any significant changes in protein conformation and biological activity. Biotinylated proteins/antibodies are widely isolated and assayed with streptavidin pull-down assay, affinity chromatography, ELISA and Western blotting etc. It is often desired to determine the degree of substitution of such biotinylated biomolecules.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Biotin Assay Buffer 1 x 25ml
    Biotin Assay Reagent A 1 x 8ml
    Biotin Assay Reagent B 1 x 1ml
    Biotin Standard Solution (10 mM in DMSO) 1 x 100µl
    Biotinylated BSA (2 mg/ml) 1 x 200µl
  • Research areas

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    • Cofactors, Vitamins / minerals
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  • Relevance

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names

    • Vitamin B4

Associated products

  • Related Buffer

    • Biotin Anti-RFP antibody (ab34771)
  • Related Products

    • Biotin Anti-BrdU antibody (ab2284)
    • Biotin Anti-GFP antibody (ab6658)

Images

  • Biotin Standard Curve
    Biotin Standard Curve

    This is example data only.

  • Amount of biotin vs amount of biotinylated BSA
    Amount of biotin vs amount of biotinylated BSA

    Linear plot obtained for the amount of biotin (nmol) vs amount of biotinylated BSA. Assays were performed according to the kit protocol. This is example data only.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab185441? Please let us know so that we can cite the reference in this datasheet.

ab185441 has been referenced in 1 publication.

  • Schoeff SS  et al. Proteomic and Genomic Methylation Signatures of Idiopathic Subglottic Stenosis. Laryngoscope 131:E540-E546 (2021). PubMed: 32619300

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