Overview

  • Product name
    Biotin Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Purified protein
  • Assay type
    Semi-quantitative
  • Sensitivity
    15 ng
  • Product overview

    Abcam’s Biotin Quantitation Kit (Colorimetric) (ab185441) is based on the differential binding of Streptavidin to Biotin and a dye, 2-(4-hydroxyazobenzene) benzoic acid (HABA). When HABA is bound to Streptavidin, addition of free biotin or biotinylated biomolecules results in its displacement in the reaction. These changes are manifested in terms of decrease in the overall absorption (OD 500 nm) and can be quantitatively correlated to the amount of biotin present in solution. The kit also provides Biotin as a Standard and Biotinylated BSA as a positive control. This kit can be used to estimate as low as 60 pmol or 15 ng of biotin in solution.

  • Notes

    Biotin (Vitamin H) is an important biomolecule that has been widely used in Biotinylation reactions to label antibodies or other proteins of interest. Due to its low molecular weight, biotin usually does not cause any significant changes in protein conformation and biological activity. Biotinylated proteins/antibodies are widely isolated and assayed with streptavidin pull-down assay, affinity chromatography, ELISA and Western blotting etc. It is often desired to determine the degree of substitution of such biotinylated biomolecules.

  • Platform
    Microplate reader

Properties

Images

  • This is example data only.

  • Linear plot obtained for the amount of biotin (nmol) vs amount of biotinylated BSA. Assays were performed according to the kit protocol. This is example data only.

Protocols

References

ab185441 has not yet been referenced specifically in any publications.

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