Key features and details
- Biotin Rabbit polyclonal to bacterial Alkaline Phosphatase
- Suitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-bacterial Alkaline Phosphatase antibody
DescriptionBiotin Rabbit polyclonal to bacterial Alkaline Phosphatase
Tested applicationsSuitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISAmore details
Species reactivityReacts with: Species independent
Full length native protein (purified) corresponding to Escherichia coli bacterial Alkaline Phosphatase.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
Concentration information loading...
Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA (1:4,000 to 1:20,000)
as well as other peroxidase-antibody based enzymatic assays.
RelevanceBacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.
- Alkaline phosphatase antibody
- APase antibody
- BAP antibody
- PhoA antibody
ab7321 has been referenced in 1 publication.
- Alcaine SD et al. Bioengineering bacteriophages to enhance the sensitivity of phage amplification-based paper fluidic detection of bacteria. Biosens Bioelectron 82:14-9 (2016). PubMed: 27031186