Key features and details
- Biotin Rabbit polyclonal to bacterial Alkaline Phosphatase
- Suitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISA
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-bacterial Alkaline Phosphatase antibody
DescriptionBiotin Rabbit polyclonal to bacterial Alkaline Phosphatase
Tested applicationsSuitable for: IHC-Fr, Immunomicroscopy, WB, Dot blot, ELISAmore details
Species reactivityReacts with: Species independent
Full length protein (E. coli).
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
Concentration information loading...
Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Our Abpromise guarantee covers the use of ab7321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA (1:4,000 to 1:20,000)
as well as other peroxidase-antibody based enzymatic assays.
RelevanceBacterial alkaline phosphatase (also known as PhoA) is derived from E. coli. It appears to require export to the periplasm to show enzymatic activity. Fusion of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Consequently, analysis of alkaline phosphatase fusions to membrane proteins of unknown structure are generally useful in determining their membrane topologies.
- Alkaline phosphatase antibody
- APase antibody
- BAP antibody
- PhoA antibody
ab7321 has not yet been referenced specifically in any publications.