Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (ab21899)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (ab21899)

    Key features and details

    • Biotin Mouse monoclonal [B2M-01] to beta 2 Microglobulin
    • Suitable for: IHC-P
    • Reacts with: Human
    • Conjugation: Biotin
    • Isotype: IgG2a

    Overview

    • Product name

      Biotin Anti-beta 2 Microglobulin antibody [B2M-01]
      See all beta 2 Microglobulin primary antibodies

    • Description

      Biotin Mouse monoclonal [B2M-01] to beta 2 Microglobulin

    • Host species

      Mouse

    • Conjugation

      Biotin

    • Specificity

      This antibody reacts with beta 2 Microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 Microglobulin.

    • Tested applications

      Suitable for: IHC-Pmore details

    • Species reactivity

      Reacts with: Human
      Does not react with: Mouse, Rabbit, Chicken, Cow, Dog

    • Immunogen

      Full length native protein (purified) (Human).

    • Positive control

      • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab21899 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use a concentration of 10 µg/ml.

    Target

    • Function

      Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

    • Involvement in disease

      Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
      Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.

    • Sequence similarities

      Belongs to the beta-2-microglobulin family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

    • Post-translational
      modifications

      Glycation of Ile-21 is observed in long-term hemodialysis patients.

    • Cellular localization

      Secreted. Detected in serum and urine.
    • Information by UniProt

    • Database links

      • Alternative names

        • B2M antibody
        • B2MG_HUMAN antibody
        • Beta 2 microglobin antibody
        • Beta 2 microglobulin antibody
        • Beta 2 microglobulin precursor antibody
        • Beta chain of mhc class 1 proteins antibody
        • Beta chain of MHC class I molecules antibody
        • Beta-2-microglobulin form pI 5.3 antibody
        • CDABP0092 antibody
        • Hdcma22p antibody
        • IMD43 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (ab21899)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (ab21899)

        IHC image of beta 2 Microglobulin staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21899, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      References (2)

      Publishing research using ab21899? Please let us know so that we can cite the reference in this datasheet.

      ab21899 has been referenced in 2 publications.

      • Zhao H  et al. A comparison of binding surfaces for SPR biosensing using an antibody-antigen system and affinity distribution analysis. Methods 59:328-35 (2013). PubMed: 23270815
      • Andersen JT  et al. FcRn binding properties of an abnormal truncated analbuminemic albumin variant. Clin Biochem 43:367-72 (2010). ELISA ; Human . PubMed: 20006594

      Customer reviews and Q&As

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      1-2 of 2 Abreviews or Q&A

      Question

      I would like to try the ab218999 for immunoprecipitation, which is the working dilution range for this assay? Thanks,

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      Answer

      Thank you for contacting us.

      When using ab21899 Anti-beta 2 Microglobulin antibody [B2M-01] (Biotin) in IP we can recommend a working range of 1-10 ug/ml. The exact concentration for your IP experiments should be within this range but could be outside of this and will need to be determined experimentally.

      Abcam also provides a number of products for use in IP such as our https://www.abcam.com/ab119211 ready to use protein gel stain, our https://www.abcam.com/index.html?pageconfig=resource&rid=15115#1 and our https://www.abcam.com/index.html?pageconfig=resource&rid=15115#2 . Our VeriBlot line only recognize native (non-reduced) antibodies and are recommended when the target of interest is <30kDa, whereas our Light Chain specific line do not recognize heavy-chains and are recommended when your protein of interest is >30kDa. All of our products are covered by our six month Abpromise guarantee and are fully supported by our scientific support staff. More information about these products can be found at the links below.

      Optiblot Blue: https://www.abcam.com/Optiblot-Blue-ab119211.html?refer404=catalogue%20119211

      IP specific secondary antibodies: https://www.abcam.com/index.html?pageconfig=resource&rid=15115


      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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      Question

      Yes, I want to coat the plate with ab94886 in order to captureHLA-G onto the plate. Then I want to detect beta 2 microglobulin(because both proteins are ensemble together in the native form) withab21889.
      So, if I want to detect these two proteins, are these clones I chose the best for ELISA? (Specially for beta 2 microglobulin, as I saw there are two distinct clones both for ELISA)

      Read More
      Answer

      These two monoclonals may be an effective pair but they have not been tested with each other in sandwich ELISA. My concern is whether or not the epitope (the binding site) that the beta 2 microglobulin antibody ab21899 recognizes is accessible if it is in an ensemble/complex with the HLA G. That is also the case for the other monoclonal, ab25160.

      A better choice may be a polyclonal antibody raised against full-length beta 2 microglobulin, which will recognize many epitopes, not just one. I suggest ab34678, a biotinylated rabbit polyclonal.

      Click here (or use the following: https://www.abcam.com/beta-2-Microglobulin-antibody-Biotin-ab34678.html).

      However, this has not been tested in sandwich ELISA with ab94886 either. We do not have any pairs that have been validated for sandwich ELISA but ab94886 and ab34678 are my recommendation. (Please note that these have only been tested against human samples).

      If you do decide to try these, we would be very interested in hearing about the result via our Abreview program. Details can be found here:

      www.abcam.com/abreviews

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