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Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (ab21899)

Submit a review Q&A (2)References (2)
Flow Cytometry - Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (ab21899)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (ab21899)

Key features and details

  • Biotin Mouse monoclonal [B2M-01] to beta 2 Microglobulin
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

PE PerCP/Cy5.5® Unconjugated

Overview

  • Product name

    Biotin Anti-beta 2 Microglobulin antibody [B2M-01]
    See all beta 2 Microglobulin primary antibodies

  • Description

    Biotin Mouse monoclonal [B2M-01] to beta 2 Microglobulin

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Specificity

    This antibody reacts with beta 2 Microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 Microglobulin.

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rabbit, Chicken, Cow, Dog

  • Immunogen

    Full length native protein (purified) (Human).

  • Positive control

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung. Flow Cytometry: Human peripheral blood.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab21899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 10 µg/ml.
Flow Cyt
Use at an assay dependent concentration.
Notes
IHC-P
Use a concentration of 10 µg/ml.
Flow Cyt
Use at an assay dependent concentration.

Target

  • Function

    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

  • Involvement in disease

    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.

  • Sequence similarities

    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    Glycation of Ile-21 is observed in long-term hemodialysis patients.

  • Cellular localization

    Secreted. Detected in serum and urine.
  • Information by UniProt

  • Database links

    • Alternative names

      • B2M antibody
      • B2MG_HUMAN antibody
      • Beta 2 microglobin antibody
      • Beta 2 microglobulin antibody
      • Beta 2 microglobulin precursor antibody
      • Beta chain of mhc class 1 proteins antibody
      • Beta chain of MHC class I molecules antibody
      • Beta-2-microglobulin form pI 5.3 antibody
      • CDABP0092 antibody
      • Hdcma22p antibody
      • IMD43 antibody
      see all

    Images

    • Flow Cytometry - Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (ab21899)
      Flow Cytometry - Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (ab21899)

      Flow cytometry analysis using anti-beta2 microglobulin (B2M-01) biotin for surface staining of beta2-microglobulin in human peripheral blood

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (ab21899)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-beta 2 Microglobulin antibody [B2M-01] (ab21899)

      IHC image of beta 2 Microglobulin staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21899, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References (2)

    Publishing research using ab21899? Please let us know so that we can cite the reference in this datasheet.

    ab21899 has been referenced in 2 publications.

    • Zhao H  et al. A comparison of binding surfaces for SPR biosensing using an antibody-antigen system and affinity distribution analysis. Methods 59:328-35 (2013). PubMed: 23270815
    • Andersen JT  et al. FcRn binding properties of an abnormal truncated analbuminemic albumin variant. Clin Biochem 43:367-72 (2010). ELISA ; Human . PubMed: 20006594

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    I would like to try the ab218999 for immunoprecipitation, which is the working dilution range for this assay? Thanks,

    Read More
    Answer

    Thank you for contacting us.

    When using ab21899 Anti-beta 2 Microglobulin antibody [B2M-01] (Biotin) in IP we can recommend a working range of 1-10 ug/ml. The exact concentration for your IP experiments should be within this range but could be outside of this and will need to be determined experimentally.

    Abcam also provides a number of products for use in IP such as our https://www.abcam.com/ab119211 ready to use protein gel stain, our https://www.abcam.com/index.html?pageconfig=resource&rid=15115#1 and our https://www.abcam.com/index.html?pageconfig=resource&rid=15115#2 . Our VeriBlot line only recognize native (non-reduced) antibodies and are recommended when the target of interest is <30kDa, whereas our Light Chain specific line do not recognize heavy-chains and are recommended when your protein of interest is >30kDa. All of our products are covered by our six month Abpromise guarantee and are fully supported by our scientific support staff. More information about these products can be found at the links below.

    Optiblot Blue: https://www.abcam.com/Optiblot-Blue-ab119211.html?refer404=catalogue%20119211

    IP specific secondary antibodies: https://www.abcam.com/index.html?pageconfig=resource&rid=15115


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Yes, I want to coat the plate with ab94886 in order to captureHLA-G onto the plate. Then I want to detect beta 2 microglobulin(because both proteins are ensemble together in the native form) withab21889.
    So, if I want to detect these two proteins, are these clones I chose the best for ELISA? (Specially for beta 2 microglobulin, as I saw there are two distinct clones both for ELISA)

    Read More
    Answer

    These two monoclonals may be an effective pair but they have not been tested with each other in sandwich ELISA. My concern is whether or not the epitope (the binding site) that the beta 2 microglobulin antibody ab21899 recognizes is accessible if it is in an ensemble/complex with the HLA G. That is also the case for the other monoclonal, ab25160.

    A better choice may be a polyclonal antibody raised against full-length beta 2 microglobulin, which will recognize many epitopes, not just one. I suggest ab34678, a biotinylated rabbit polyclonal.

    Click here (or use the following: https://www.abcam.com/beta-2-Microglobulin-antibody-Biotin-ab34678.html).

    However, this has not been tested in sandwich ELISA with ab94886 either. We do not have any pairs that have been validated for sandwich ELISA but ab94886 and ab34678 are my recommendation. (Please note that these have only been tested against human samples).

    If you do decide to try these, we would be very interested in hearing about the result via our Abreview program. Details can be found here:

    www.abcam.com/abreviews

    Read More

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