Key features and details
- Biotin Mouse monoclonal [B2M-01] to beta 2 Microglobulin
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2a
Product nameBiotin Anti-beta 2 Microglobulin antibody [B2M-01]
See all beta 2 Microglobulin primary antibodies
DescriptionBiotin Mouse monoclonal [B2M-01] to beta 2 Microglobulin
SpecificityThis antibody reacts with beta 2 Microglobulin associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta 2 Microglobulin.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Does not react with: Mouse, Rabbit, Chicken, Cow, Dog
Full length native protein (purified) (Human).
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab21899 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml.|
FunctionComponent of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
Involvement in diseaseDefects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.
Sequence similaritiesBelongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
modificationsGlycation of Ile-21 is observed in long-term hemodialysis patients.
Cellular localizationSecreted. Detected in serum and urine.
- Information by UniProt
- B2M antibody
- B2MG_HUMAN antibody
- Beta 2 microglobin antibody
IHC image of beta 2 Microglobulin staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21899, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab21899 has been referenced in 2 publications.
- Zhao H et al. A comparison of binding surfaces for SPR biosensing using an antibody-antigen system and affinity distribution analysis. Methods 59:328-35 (2013). PubMed: 23270815
- Andersen JT et al. FcRn binding properties of an abnormal truncated analbuminemic albumin variant. Clin Biochem 43:367-72 (2010). ELISA ; Human . PubMed: 20006594