Key features and details
- Biotin Rabbit polyclonal to beta 5 Defensin
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-beta 5 Defensin antibody
DescriptionBiotin Rabbit polyclonal to beta 5 Defensin
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Human, Chimpanzee, Gorilla, Orangutan
- WB: Recombinant human beta 5 Defensin protein. sELISA: Recombinant human beta 5 Defensin protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Reconstitute in sterile PBS + 0.1% BSA to 0.1 - 1.0mg/mL.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab246314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.|
RelevanceDefensins (a and ß) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The a-defensins are distinguished from the ß-defensins by the pairing of their three disulfide bonds. To date, six human ß-defensins have been identified; BD-1, BD-2, BD-3, BD-4, BD-5 and BD-6. ß-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they can act as chemoattractants towards immature dendritic cells and memory T cells. The ß-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and in some cases, a propeptide sequence. ß-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds.
- BD5 antibody
- Beta defensin 105 precursor antibody
- DEFB105 antibody
To detect human beta 5 Defensin by Western Blot analysis ab246314 can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hhuman beta 5 Defensin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lanes 1-11: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human beta 5 Defensin, respectively.
To detect human beta 5 Defensin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab246314 is required. This biotinylated polyclonal antibody, in conjunction with a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human beta 5 Defensin.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab246314 has not yet been referenced specifically in any publications.