Recombinant Biotin Anti-beta Actin antibody [RM112] - N-terminal (ab222760)
![Western blot - Biotin Anti-beta Actin antibody [RM112] - N-terminal (ab222760)](https://www.abcam.com/ps/products/222/ab222760/Images/ab222760-282521-anti-beta-actin-antibody-rm112-n-terminal-biotin-western-blot.jpg "Western blot - Biotin Anti-beta Actin antibody [RM112] - N-terminal (ab222760)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Biotin Rabbit monoclonal [RM112] to beta Actin - N-terminal
- Suitable for: ICC/IF, WB
- Conjugation: Biotin
Overview
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Product name
Biotin Anti-beta Actin antibody [RM112] - N-terminal
See all beta Actin primary antibodies -
Description
Biotin Rabbit monoclonal [RM112] to beta Actin - N-terminal -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
No cross reactivity with other isoforms of Actin. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Predicted to work with: a wide range of other species
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Immunogen
Synthetic peptide corresponding to beta Actin (N terminal).
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Positive control
- ICC/IF: HeLa (human epithelial cell line from cervix adenocarcinoma) cells. WB: A431 (human epidermoid carcinoma cell line) cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM112 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab222760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/200. | |
| WB | 1/1000. |
Target
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Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
Involvement in disease
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
Sequence similarities
Belongs to the actin family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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Alternative names
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
Images
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Western blot - Biotin Anti-beta Actin antibody [RM112] - N-terminal (ab222760)Biotin Anti-beta Actin antibody [RM112] - N-terminal (ab222760) at 1/1000 dilution + A431 cell lysate
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![Immunocytochemistry/ Immunofluorescence - Biotin Anti-beta Actin antibody [RM112] - N-terminal (ab222760)](https://www.abcam.com/ps/products/222/ab222760/Images/ab222760-282520-anti-beta-actin-antibody-rm112-n-terminal-biotin-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Biotin Anti-beta Actin antibody [RM112] - N-terminal (ab222760)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin (red) using ab222760 at a 1/200 dilution in ICC/IF. Nuclei counterstain DAPI (blue).
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![Biotin Anti-beta Actin antibody [RM112] - N-terminal (ab222760)](https://www.abcam.com/ps/products/222/ab222760/Images/ab222760-3-benefits-of-recombinant-antibodies.png)
Biotin Anti-beta Actin antibody [RM112] - N-terminal (ab222760)
Protocols
Datasheets and documents
References (0)
ab222760 has not yet been referenced specifically in any publications.
