Key features and details
- Biotin Rabbit polyclonal to beta Defensin 1
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-beta Defensin 1 antibody
See all beta Defensin 1 primary antibodies
DescriptionBiotin Rabbit polyclonal to beta Defensin 1
Tested applicationsSuitable for: Sandwich ELISA, WBmore details
Species reactivityReacts with: Human
Highly pure (>98%) recombinant Human beta Defensin 1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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FormLyophilized:Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab84245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.
Use 100 μl/well antibody solution. Detects of at least 0.2 – 0.4 ng/well of recombinant human beta Defensin 1.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 8 kDa.
The detection limit for recombinant human beta Defensin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
FunctionHas bactericidal activity.
Sequence similaritiesBelongs to the beta-defensin family.
- Information by UniProt
- BD 1 antibody
- BD-1 antibody
- BD1 antibody
To detect human beta Defensin 1 by Western Blot analysis ab84245 can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human beta Defensin 1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Human recombinant beta Defensin 1 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant beta Defensin 1 protein.
To detect human beta Defensin 1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab84245 is required. This biotinylated polyclonal antibody, in conjunction with an anti-beta Defensin 1 (ab109571) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human beta Defensin 1.
ab84245 has been referenced in 1 publication.
- Trend S et al. Antimicrobial protein and Peptide concentrations and activity in human breast milk consumed by preterm infants at risk of late-onset neonatal sepsis. PLoS One 10:e0117038 (2015). ELISA . PubMed: 25643281