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  1. Link

    biotin-beta-tubulin-antibody-bt7r-ab173839.pdf

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Isotype/Loading Controls Loading Controls Tubulin
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Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)

  • Datasheet
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Abpromise

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Western blot - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)
  • Immunocytochemistry/ Immunofluorescence - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)
  • Flow Cytometry - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)
  • Flow Cytometry - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)
  • Flow Cytometry - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)

Key features and details

  • Biotin Mouse monoclonal [BT7R] to beta Tubulin
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Non human primates
  • Conjugation: Biotin
  • Isotype: IgG2a

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Overview

  • Product name

    Biotin Anti-beta Tubulin antibody [BT7R]
    See all beta Tubulin primary antibodies
  • Description

    Biotin Mouse monoclonal [BT7R] to beta Tubulin
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Non human primates
  • Immunogen

    Synthetic peptide corresponding to Human beta Tubulin (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P07437

  • Positive control

    • HeLa, 293T, COS7, NRK and C2C12 cell line lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    BT7R
  • Isotype

    IgG2a
  • Research areas

    • Isotype/Loading Controls
    • Loading Controls
    • Tubulin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Isotype/Loading Controls
    • Loading Controls
    • Beta Tubulin

Associated products

  • Alternative Versions

    • HRP Anti-beta Tubulin antibody [BT7R] (ab173840)
  • Isotype control

    • Biotin Mouse IgG2a, kappa [MOPC-173] - Isotype Control (ab18454)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human beta Tubulin protein (ab70187)
  • Related Products

    • Recombinant Human beta Tubulin protein (ab70187)

Applications

Our Abpromise guarantee covers the use of ab173839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2000.
Flow Cyt Use at an assay dependent concentration.

1 μg/test. ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.

Target

  • Function

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Tissue specificity

    Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
  • Involvement in disease

    Cortical dysplasia, complex, with other brain malformations 6
    Skin creases, congenital symmetric circumferential, 1
  • Sequence similarities

    Belongs to the tubulin family.
  • Domain

    The highly acidic C-terminal region may bind cations such as calcium.
  • Post-translational
    modifications

    Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
    Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
    Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
  • Cellular localization

    Cytoplasm, cytoskeleton.
  • Target information above from: UniProt accession P07437 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396254 Chicken
    • Entrez Gene: 203068 Human
    • Entrez Gene: 22154 Mouse
    • Entrez Gene: 29214 Rat
    • Omim: 191130 Human
    • SwissProt: P07437 Human
    • SwissProt: P99024 Mouse
    • SwissProt: P69897 Rat
    • Unigene: 636480 Human
    see all
  • Alternative names

    • Beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • beta Ib tubulin antibody
    • Beta1 tubulin antibody
    • Class I beta tubulin antibody
    • M40 antibody
    • MGC117247 antibody
    • MGC16435 antibody
    • OK/SW cl.56 antibody
    • OK/SWcl.56 antibody
    • TBB5_HUMAN antibody
    • TUBB 1 antibody
    • TUBB 2 antibody
    • TUBB 5 antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin beta 1 chain antibody
    • Tubulin beta 2 chain antibody
    • tubulin beta 5 chain antibody
    • Tubulin beta chain antibody
    • Tubulin beta class I antibody
    • tubulin beta polypeptide antibody
    • Tubulin beta-5 chain antibody
    see all

Images

  • Western blot - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)
    Western blot - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)
    All lanes : Biotin Anti-beta Tubulin antibody [BT7R] (ab173839) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : COS7 cell lysate
    Lane 5 : C2C12 cell lysate
    Lane 6 : NRK cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Streptavidin-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 50 kDa



    4-20% Tris-HCl polyacrylamide gel.

  • Immunocytochemistry/ Immunofluorescence - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)
    Immunocytochemistry/ Immunofluorescence - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)

    Immunofluorescent analysis of Beta-Tubulin (red) in HEK293T cells. Cells fixed in 4% formaldehyde were permeabilized and blocked with 1X PBS containing 5% BSA and 0.3% Triton X-100 for 1 hour at room temperature. Cells were probed with a Beta-Tubulin monoclonal antibody (ab173839) at a dilution of 1:100 overnight at 4ºC in 1X PBS containing 1% BSA and 0.3% Triton X-100, washed with 1X PBS, and incubated with a fluorophore-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:200 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI. Images were taken  at 40X magnification.

  • Flow Cytometry - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)
    Flow Cytometry - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)

    Flow cytometry analysis of Beta Tubulin in CEM cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Beta Tubulin loading control antibody (ab173839) at a dilution of 1 ug/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow Cytometry - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)
    Flow Cytometry - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)

    Flow cytometry analysis of Beta Tubulin in Hela cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Beta Tubulin loading control antibody (ab173839) at a dilution of 1 ug/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow Cytometry - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)
    Flow Cytometry - Biotin Anti-beta Tubulin antibody [BT7R] (ab173839)

    Flow cytometry analysis of Beta Tubulin in NIH-3T3 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Beta Tubulin loading control antibody (ab173839) at a dilution of 1 ug/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab173839? Please let us know so that we can cite the reference in this datasheet.

    ab173839 has not yet been referenced specifically in any publications.

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