Biotin Anti-BrdU antibody (ab2284)
Key features and details
- Biotin Sheep polyclonal to BrdU
- Suitable for: IHC-P, IHC-Fr, ICC/IF, ELISA, IHC-FoFr
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-BrdU antibody
See all BrdU primary antibodies -
Description
Biotin Sheep polyclonal to BrdU -
Host species
Sheep -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-P, IHC-Fr, ICC/IF, ELISA, IHC-FoFrmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH)
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituent: 0.4% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Related Buffer
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Related Products
Applications
Our Abpromise guarantee covers the use of ab2284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. Fixation in cold methanol for 30 minutes followed by immersion in 7 x 10-3 N NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail. | |
| ICC/IF | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. dilute from 1/500 to 1/40,000 against 1mg/mL BrdU analyte. | |
| IHC-FoFr | 1/2000. 1/2000 (see Abreview). |
Target
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Relevance
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
Cellular localization
Nuclear -
Alternative names
- Bromodeoxyuridine antibody
- BUdr antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (ab2284)This image is courtesy of an anonymous Abreview
ab2284 staining BrdU in mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% H2O2 in methanol for 12 minutes; antigen retrieval was by heat mediation in 10mM citrate, pH6. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C.
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Immunocytochemistry/ Immunofluorescence - Biotin Anti-BrdU antibody (ab2284)This image is courtesy of an anonymous Abreviewab2284 at 1/250 staining primary E12 mouse cortex cells by ICC/IF. The cells were paraformaldehyde fixed, blocked with serum and then incubated with the antibody for 24 hours. Streptavidin conjugated to Alexa-Fluor ® 488 was used as the secondary. The image shows BrdU staining with nuclei counterstained with DAPI.
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Immunohistochemistry (Frozen sections) - Biotin Anti-BrdU antibody (ab2284)This image is courtesy of an Abreview submitted by Ahmar Azizab2284 staining BrdU in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 30 minutes. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 4°C. A Streptavidin Alexa Fluor® 488-conjugated Goat polyclonal (1/500) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (ab2284)This image is courtesy of an Abreview submitted by Dr Christoph Schwarzerab2284 at 1/2000 dilution staining mouse free floating brain slices by Immunohistochemistry (Formalin/PFA fixed sections). The mice were treated with 100mg/kg BrdU 2 hours before fixation. Free floating 40µm vibratome sections were obtained from paraformaldehyde fixed brains, these were incubated with the antibody for 24 hours. A streptavidin-HRP complex and DAB were used for detection. The image depicts the subventricular zone.
Datasheets and documents
References (42)
ab2284 has been referenced in 42 publications.
- Fu DJ et al. Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5-CD44+ cells. Nat Commun 11:84 (2020). PubMed: 31901081
- Xiao Y et al. LncRNA MALAT1 increases the stemness of gastric cancer cells via enhancing SOX2 mRNA stability. FEBS Open Bio 9:1212-1222 (2019). PubMed: 31037832
- Flentke GR et al. Alcohol-mediated calcium signals dysregulate pro-survival Snai2/PUMA/Bcl2 networks to promote p53-mediated apoptosis in avian neural crest progenitors. Birth Defects Res N/A:N/A (2019). PubMed: 31021056
- Razumilava N et al. Hedgehog Signaling Modulates Interleukin-33-Dependent Extrahepatic Bile Duct Cell Proliferation in Mice. Hepatol Commun 3:277-292 (2019). PubMed: 30766964
- Campanale JP et al. Methods to label, isolate, and image sea urchin small micromeres, the primordial germ cells (PGCs). Methods Cell Biol 150:269-292 (2019). PubMed: 30777180
