Biotin Anti-BrdU antibody (ab2284)
Key features and details
- Biotin Sheep polyclonal to BrdU
- Suitable for: IHC-P, IHC-Fr, ICC/IF, ELISA, IHC-FoFr
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-BrdU antibody
See all BrdU primary antibodies -
Description
Biotin Sheep polyclonal to BrdU -
Host species
Sheep -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-P, IHC-Fr, ICC/IF, ELISA, IHC-FoFrmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to BrdU conjugated to keyhole limpet haemocyanin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituent: 0.4% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Related Buffer
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
Use at an assay dependent concentration.
|
| IHC-Fr | (1) |
Use at an assay dependent concentration. Fixation in cold methanol for 30 minutes followed by immersion in 7 x 10-3 N NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration. dilute from 1/500 to 1/40,000 against 1mg/mL BrdU analyte.
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| IHC-FoFr | (1) |
1/2000. 1/2000 (see Abreview).
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. Fixation in cold methanol for 30 minutes followed by immersion in 7 x 10-3 N NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail. |
|
ICC/IF
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. dilute from 1/500 to 1/40,000 against 1mg/mL BrdU analyte. |
|
IHC-FoFr
1/2000. 1/2000 (see Abreview). |
Target
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Relevance
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
Cellular localization
Nuclear -
Alternative names
- Bromodeoxyuridine antibody
- BUdr antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (ab2284)This image is courtesy of an anonymous Abreview
ab2284 staining BrdU in mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% H2O2 in methanol for 12 minutes; antigen retrieval was by heat mediation in 10mM citrate, pH6. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C.
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Immunocytochemistry/ Immunofluorescence - Biotin Anti-BrdU antibody (ab2284)This image is courtesy of an anonymous Abreviewab2284 at 1/250 staining primary E12 mouse cortex cells by ICC/IF. The cells were paraformaldehyde fixed, blocked with serum and then incubated with the antibody for 24 hours. Streptavidin conjugated to Alexa-Fluor ® 488 was used as the secondary. The image shows BrdU staining with nuclei counterstained with DAPI.
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Immunohistochemistry (Frozen sections) - Biotin Anti-BrdU antibody (ab2284)This image is courtesy of an Abreview submitted by Ahmar Azizab2284 staining BrdU in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 30 minutes. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 4°C. A Streptavidin Alexa Fluor® 488-conjugated Goat polyclonal (1/500) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (ab2284)This image is courtesy of an Abreview submitted by Dr Christoph Schwarzerab2284 at 1/2000 dilution staining mouse free floating brain slices by Immunohistochemistry (Formalin/PFA fixed sections). The mice were treated with 100mg/kg BrdU 2 hours before fixation. Free floating 40µm vibratome sections were obtained from paraformaldehyde fixed brains, these were incubated with the antibody for 24 hours. A streptavidin-HRP complex and DAB were used for detection. The image depicts the subventricular zone.
Datasheets and documents
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Datasheet download
References (47)
ab2284 has been referenced in 47 publications.
- Bühler L et al. Lipocalin 13 enhances insulin secretion but is dispensable for systemic metabolic control. Life Sci Alliance 4:N/A (2021). PubMed: 33536239
- Lamarche É et al. SMAD2 promotes myogenin expression and terminal myogenic differentiation. Development 148:N/A (2021). PubMed: 33462116
- Fu DJ et al. Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5-CD44+ cells. Nat Commun 11:84 (2020). PubMed: 31901081
- Freria CM et al. Serial Systemic Injections of Endotoxin (LPS) Elicit Neuroprotective Spinal Cord Microglia through IL-1-Dependent Cross Talk with Endothelial Cells. J Neurosci 40:9103-9120 (2020). PubMed: 33051350
- Hughes KR et al. The early life microbiota protects neonatal mice from pathological small intestinal epithelial cell shedding. FASEB J 34:7075-7088 (2020). PubMed: 32253791
