Biotin Anti-C10 antibody (ab83134)

Submit a review Q&A (2)

Key features and details

  • Biotin Rabbit polyclonal to C10
  • Reacts with: Mouse
  • Conjugation: Biotin
  • Isotype: IgG

Overview

  • Product name

    Biotin Anti-C10 antibody
    See all C10 primary antibodies

  • Description

    Biotin Rabbit polyclonal to C10

  • Host species

    Rabbit

  • Conjugation

    Biotin

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant full length protein corresponding to Mouse C10.

  • General notes

    Protein target previously labeled as CCL6.

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    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

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    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    ab83134 is sterile filtered.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Target

  • Relevance

    CCL6 (C10) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. It is a potent chemoattractant of macrophages, but it can also attract B cells, CD4+ lymphocytes and eosinophils. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be induced under conditions suitable for myeloid cell differentiation. CCL6 (C10) was originally identified as a transcript that is induced in bone marrow cells upon stimulation of GMCSF. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

  • Cellular localization

    Secreted

  • Database links

    • Alternative names

      • C10 antibody
      • CCL 6 antibody
      • chemokine (C-C motif) ligand 6 antibody
      • Chemokine C C motif Ligand 6 antibody
      • MRP1 antibody
      • SCYA6 antibody
      • Small Inducible Cytokine A6 antibody
      see all

    References (0)

    Publishing research using ab83134? Please let us know so that we can cite the reference in this datasheet.

    ab83134 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    My question is whether the "highly pure recombinant mouse CCL6" you used to immunize the animals is:
    (1) complete full length protein containing N-terminal signal peptide (a.a 1-21)?,
    (2) secreted protein lacking signal peptide but maintain whole other parts (a.a 22-116)? or
    (3) mature active protein lacking the N-terminal domain (22-43), which contains only a.a. 44-116?
    I can agree with your statement that epitope mapping is usually not performed for validating poly-clonal antibodies.
    So the details of the immunogen is quite important to estimate the reactivity of the antibody.
    If (3) above is the case, we don't have to worry about the reactivity because this antibody must react with all forms of CCL6.
    But if (1) or (2) is the case, it might happen that this antibody preferentially react with inactive form of CCL6 with N-terminal a.a. 1-43 or 22-43.
    So if possible, please give me the details of recombinant CCL6 used as an immunogen.
    Regards,

    Read More
    Answer

    Thank you for your enquiry.

    The recombinant MurineCCL6 (C10) is also in our catalogue ab9898. This is the mature protein, which contains 95 amino acids (amino acids 22-116).

    The amino acid sequence is as follows:

    GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA

    The 21 amino acid N-terminal signal peptide is not present on the protein.

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Question

    Lot number: GR82949-1
    Inquiry: We purchased the antibody above (anti-CCL6 biotin) for WB, and could see some signals in our samples using this antibody. But the band size we saw was significantly larger than the expected. Since CCL6 is processed from its inactive form (pro-peptide) to the active mature form, we assume that the band we saw was the inactive pro-peptide. We want to know if the antibody could also detect the active form of CCL6, to know if our samples contain active CCL6. For this, we ask two specific questions: (1) The immunogen used to raise this antibody is the active (truncated) CCL6 or full-length inactive CCL6? (2) If full length you used, have you confirmed epitopes this antibody recognizes? Many thanks

    Read More
    Answer

    Thank you for your enquiry regarding ab83134.

    The immunogen used to raise this antibody was a highly pure (>98%) recombinant mouse CCL6. This is a polyclonal antibody and will therefore consist of a mixed population of IgGs each of which will recognize a different epitope. For polyclonal antibodies epitope mapping experiment is not carried out.

    Currently, as our datasheet indicates we have no data with this product on cell/tissue samples yet this needs to be tested.

    If you need any further assistance or help, please do not hesitate to contact me.

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