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Cell Biology Apoptosis Intracellular Caspases etc Caspases
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RecombinantRabMAb

Recombinant Biotin Anti-Caspase-3 antibody [E87] (ab195905)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Caspase-3 antibody [E87] (ab195905)
  • Western blot - Biotin Anti-Caspase-3 antibody [E87] (ab195905)
  • Biotin Anti-Caspase-3 antibody [E87] (ab195905)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Biotin Rabbit monoclonal [E87] to Caspase-3
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment
  • Conjugation: Biotin

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Overview

  • Product name

    Biotin Anti-Caspase-3 antibody [E87]
    See all Caspase-3 primary antibodies
  • Description

    Biotin Rabbit monoclonal [E87] to Caspase-3
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Synthetic peptide within Human Caspase-3 aa 50-150. The exact sequence is proprietary.
    Database link: P42574

  • Positive control

    • WB: Active Caspase-3 recombinant protein. IHC-P: Human normal tonsil (FFPE sections)
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E87
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism
    • Signal Transduction
    • Antibodies
    • caspase-3

Associated products

  • Alternative Versions

    • Anti-Caspase-3 antibody [E87] - BSA and Azide free (ab197202)
    • Anti-Caspase-3 antibody [E87] (ab32351)
  • Isotype control

    • Biotin Rabbit IgG - Isotype Control (ab200208)
  • Positive Controls

    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human Caspase-3 protein (BSA and azide free) (denatured) (ab180336)

Applications

Our Abpromise guarantee covers the use of ab195905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 15 kDa (predicted molecular weight: 31 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
  • Tissue specificity

    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similarities

    Belongs to the peptidase C14A family.
  • Post-translational
    modifications

    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P42574 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 836 Human
    • Omim: 600636 Human
    • SwissProt: P42574 Human
    • Unigene: 141125 Human
    • Alternative names

      • A830040C14Rik antibody
      • Apopain antibody
      • CASP 3 antibody
      • CASP-3 antibody
      • CASP3 antibody
      • CASP3_HUMAN antibody
      • Casp3a antibody
      • Caspase 3 antibody
      • Caspase 3, apoptosis-related cysteine peptidase antibody
      • Caspase 3, apoptosis-related cysteine protease antibody
      • Caspase 3, apoptosis-related cysteine protease a antibody
      • Caspase-3 subunit p12 antibody
      • Caspase3 antibody
      • CC3 antibody
      • CPP 32 antibody
      • CPP-32 antibody
      • CPP32 antibody
      • CPP32B antibody
      • Cysteine protease CPP32 antibody
      • EC 3.4.22.56 antibody
      • ICE3 antibody
      • LICE antibody
      • mldy antibody
      • OTTHUMP00000165052 antibody
      • OTTHUMP00000165053 antibody
      • OTTHUMP00000165054 antibody
      • PARP cleavage protease antibody
      • Procaspase3 antibody
      • Protein Yama antibody
      • SCA 1 antibody
      • SCA-1 antibody
      • SCA1 antibody
      • SREBP cleavage activity 1 antibody
      • Yama antibody
      • Yama protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Caspase-3 antibody [E87] (ab195905)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Caspase-3 antibody [E87] (ab195905)

      IHC image of Caspase-3 staining in a section of formalin-fixed paraffin-embedded Human normal tonsil*, performed on a Leica BondTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins, before blocking of endogenous biotin using ab64212. The section was then incubated with ab195905, 1/50 dilution, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - Biotin Anti-Caspase-3 antibody [E87] (ab195905)
      Western blot - Biotin Anti-Caspase-3 antibody [E87] (ab195905)
      Biotin Anti-Caspase-3 antibody [E87] (ab195905) at 1/10000 dilution + Recombinant human Cleaved Caspase-3 protein (Active) (ab52101) at 0.1 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 31 kDa
      Observed band size: 15 kDa
      why is the actual band size different from the predicted?


      Exposure time: 10 seconds


      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195905 overnight at 4°C. Antibody binding to the target was detected using a HRP conjugated streptavidin amplification step, and visualised using ECL development solution ab133406.

    • Biotin Anti-Caspase-3 antibody [E87] (ab195905)
      Biotin Anti-Caspase-3 antibody [E87] (ab195905)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab195905? Please let us know so that we can cite the reference in this datasheet.

    ab195905 has not yet been referenced specifically in any publications.

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