Biotin Anti-CD11b + CD11c antibody [OX42] (ab33827)

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Flow Cytometry - Biotin Anti-CD11b + CD11c antibody [OX42] (ab33827)

    Key features and details

    • Biotin Mouse monoclonal [OX42] to CD11b + CD11c
    • Suitable for: Flow Cyt
    • Reacts with: Rat
    • Conjugation: Biotin
    • Isotype: IgG2b

    Overview

    • Product name

      Biotin Anti-CD11b + CD11c antibody [OX42]

    • Description

      Biotin Mouse monoclonal [OX42] to CD11b + CD11c

    • Host species

      Mouse

    • Conjugation

      Biotin

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Rat

    • Immunogen

      Tissue, cells or virus corresponding to Rat CD11b. Resident peritoneal macrophages from (PVG.RT1[c] x PVG.RT1[u]) and (PVG.RT1[c] x PVG.RT1[a]) F1-hybrid rat.

    • Positive control

      • Rat peritoneal macrophages

    • General notes

      ab33827 recognises CD11b + CD11c expressed on most macrophages, including resident and activated peritoneal macrophages, Kupffer cells, around 35% of alveolar macrophages, dendritic cells, granulocytes and microglial cells in the brain.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab33827 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 10µl for 106 cells.

    Use neat - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl.

     

     

     

     

    ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

     

    Target

    Images

    • Flow Cytometry - Biotin Anti-CD11b + CD11c antibody [OX42] (ab33827)
      Flow Cytometry - Biotin Anti-CD11b + CD11c antibody [OX42] (ab33827)
      FACS analysis of rat peritoneal macrophages using ab33827.

    References (4)

    Publishing research using ab33827? Please let us know so that we can cite the reference in this datasheet.

    ab33827 has been referenced in 4 publications.

    • Xie Y  et al. Electro-acupuncture stimulation prevents remifentanil-induced postoperative hyperalgesia by suppressing spinal microglia in rats. Exp Ther Med 16:353-359 (2018). PubMed: 29896261
    • Tsuruta T  et al. Dental pulp-derived stem cell conditioned medium to regenerate peripheral nerves in a novel animal model of dysphagia. PLoS One 13:e0208938 (2018). PubMed: 30533035
    • Uyangaa E  et al. Dual TLR2/9 Recognition of Herpes Simplex Virus Infection Is Required for Recruitment and Activation of Monocytes and NK Cells and Restriction of Viral Dissemination to the Central Nervous System. Front Immunol 9:905 (2018). PubMed: 29760708
    • Clements MP  et al. The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. Neuron 96:98-114.e7 (2017). PubMed: 28957681

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Can a biotin-conjugated Ab be detected using streptavidin-HRP?

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    Answer

    Yes, a biotin-conjugated Ab can be readily detected via streptavidin conjugated to HRP. The biotin-streptavidin interaction is very specific and of very high affinity.

    One option for a ready-to-use streptavidin-peroxidase product can be found here: Streptavidin Peroxidase (Ready to Use). Read More

    Question

    Can you provide any recommendations or references for use of an anti-CD11b Ab in with fluorescent staining methods?

    Are specific fixation methods recommended?

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    Answer

    The following references report use of Ab clone OX42 in fluorescent staining applications:
    Stidworthy MF, et al. Notch1 and Jagged1 are expressed after CNS demyelination, but are not a major rate-determining factor during remyelination. Brain. 2004 Sep;127(Pt 9):1928-41. Epub 2004 Aug 2. PMID: 15289265.

    We would generally recommend acetone fixation for frozen sections, as used in the publication below:
    Lehrmann E, et al. Microglial and macrophage reactions mark progressive changes and define the penumbra in the rat neocortex and striatum after transient middle cerebral artery occlusion. J Comp Neurol. 1997 Sep 29;386(3):461-76. PMID: 9303429.

    The reference by Milligan et al. would suggest that paraformaldehyde fixation is not suitable for this antibody; however, please note that they used PLP fixed tissues successfully:
    Milligan CE, et al. Differential immunochemical markers reveal the normal distribution of brain macrophages and microglia in the developing rat brain. J Comp Neurol. 1991 Dec 1;314(1):125-35. PMID: 1797868.

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