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    biotin-cd11b--cd11c-antibody-ox42-ab33827.pdf

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Neuroscience Cell Adhesion Proteins Receptors Integrins
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Biotin Anti-CD11b + CD11c antibody [OX42] (ab33827)

  • Datasheet
Submit a review Q&A (2)References (5)

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Flow Cytometry - Biotin Anti-CD11b + CD11c antibody [OX42] (ab33827)

    Key features and details

    • Biotin Mouse monoclonal [OX42] to CD11b + CD11c
    • Suitable for: Flow Cyt
    • Reacts with: Rat
    • Conjugation: Biotin
    • Isotype: IgG2b

    Conjugates logo Related conjugates and formulations

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    Overview

    • Product name

      Biotin Anti-CD11b + CD11c antibody [OX42]
    • Description

      Biotin Mouse monoclonal [OX42] to CD11b + CD11c
    • Host species

      Mouse
    • Conjugation

      Biotin
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Rat
    • Immunogen

      Tissue, cells or virus corresponding to Rat CD11b. Resident peritoneal macrophages from (PVG.RT1[c] x PVG.RT1[u]) and (PVG.RT1[c] x PVG.RT1[a]) F1-hybrid rat.

    • Positive control

      • Rat peritoneal macrophages
    • General notes

      ab33827 recognises CD11b + CD11c expressed on most macrophages, including resident and activated peritoneal macrophages, Kupffer cells, around 35% of alveolar macrophages, dendritic cells, granulocytes and microglial cells in the brain.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Protein G purified
    • Primary antibody notes

      ab33827 recognises CD11b expressed on most macrophages, including resident and activated peritoneal macrophages, Kupffer cells, around 35% of alveolar macrophages, dendritic cells, granulocytes and microglial cells in the brain.
    • Clonality

      Monoclonal
    • Clone number

      OX42
    • Isotype

      IgG2b
    • Research areas

      • Neuroscience
      • Cell Adhesion Proteins
      • Receptors
      • Integrins
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cell Adhesion
      • Integrins
      • Alpha
      • Tags & Cell Markers
      • Cell Type Markers
      • Neuroscience Markers
      • Glial
      • Immunology
      • Cell Type Markers
      • CD
      • Adhesion
      • Neuroscience
      • Cell Type Marker
      • Glia marker
      • Microglia marker
      • Stem Cells
      • Mesenchymal Stem Cells
      • Negative Markers
      • Stem Cells
      • Hematopoietic Progenitors
      • Hematopoietic Stem Cells
      • Murine Lineage Negative
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • NK Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Dendritic Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Neutrophil Lineage

    Associated products

    • Alternative Versions

      • Anti-CD11b + CD11c antibody [OX42] (ab1211)
      • Alexa Fluor® 488 Anti-CD11b + CD11c antibody [OX42] (ab216355)
      • Alexa Fluor® 647 Anti-CD11b + CD11c antibody [OX42] (ab216524)
      • Anti-CD11b + CD11c antibody [OX42] - BSA and Azide free (ab238658)
    • Isotype control

      • Biotin Mouse IgG2b, kappa monoclonal [MPC-11] - Isotype Control (ab18418)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab33827 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 10µl for 106 cells.

    Use neat - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl.

     

     

     

     

    ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    Use 10µl for 106 cells.

    Use neat - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl.

     

     

     

     

    ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

     

    Target

    • Cellular localization

      CD11b: Membrane. CD11c: Membrane.
    • Database links

      • Entrez Gene: 25021 Rat
      • Entrez Gene: 499271 Rat

      Images

      • Flow Cytometry - Biotin Anti-CD11b + CD11c antibody [OX42] (ab33827)
        Flow Cytometry - Biotin Anti-CD11b + CD11c antibody [OX42] (ab33827)
        FACS analysis of rat peritoneal macrophages using ab33827.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (5)

      Publishing research using ab33827? Please let us know so that we can cite the reference in this datasheet.

      ab33827 has been referenced in 5 publications.

      • Shi Y  et al. Down Syndrome Critical Region 1 Reduces Oxidative Stress-Induced Retinal Ganglion Cells Apoptosis via CREB-Bcl-2 Pathway. Invest Ophthalmol Vis Sci 61:23 (2020). PubMed: 33104163
      • Xie Y  et al. Electro-acupuncture stimulation prevents remifentanil-induced postoperative hyperalgesia by suppressing spinal microglia in rats. Exp Ther Med 16:353-359 (2018). PubMed: 29896261
      • Tsuruta T  et al. Dental pulp-derived stem cell conditioned medium to regenerate peripheral nerves in a novel animal model of dysphagia. PLoS One 13:e0208938 (2018). PubMed: 30533035
      • Uyangaa E  et al. Dual TLR2/9 Recognition of Herpes Simplex Virus Infection Is Required for Recruitment and Activation of Monocytes and NK Cells and Restriction of Viral Dissemination to the Central Nervous System. Front Immunol 9:905 (2018). PubMed: 29760708
      • Clements MP  et al. The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. Neuron 96:98-114.e7 (2017). PubMed: 28957681

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-2 of 2 Abreviews or Q&A

      Question

      Can a biotin-conjugated Ab be detected using streptavidin-HRP?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 22 2013

      Answer

      Yes, a biotin-conjugated Ab can be readily detected via streptavidin conjugated to HRP. The biotin-streptavidin interaction is very specific and of very high affinity.

      One option for a ready-to-use streptavidin-peroxidase product can be found here: Streptavidin Peroxidase (Ready to Use). Read More

      Abcam Scientific Support

      Answered on Jan 22 2013

      Question

      Can you provide any recommendations or references for use of an anti-CD11b Ab in with fluorescent staining methods?

      Are specific fixation methods recommended?

      Read More

      Abcam community

      Verified customer

      Asked on May 01 2007

      Answer

      The following references report use of Ab clone OX42 in fluorescent staining applications:
      Stidworthy MF, et al. Notch1 and Jagged1 are expressed after CNS demyelination, but are not a major rate-determining factor during remyelination. Brain. 2004 Sep;127(Pt 9):1928-41. Epub 2004 Aug 2. PMID: 15289265.

      We would generally recommend acetone fixation for frozen sections, as used in the publication below:
      Lehrmann E, et al. Microglial and macrophage reactions mark progressive changes and define the penumbra in the rat neocortex and striatum after transient middle cerebral artery occlusion. J Comp Neurol. 1997 Sep 29;386(3):461-76. PMID: 9303429.

      The reference by Milligan et al. would suggest that paraformaldehyde fixation is not suitable for this antibody; however, please note that they used PLP fixed tissues successfully:
      Milligan CE, et al. Differential immunochemical markers reveal the normal distribution of brain macrophages and microglia in the developing rat brain. J Comp Neurol. 1991 Dec 1;314(1):125-35. PMID: 1797868.

      Read More

      Abcam Scientific Support

      Answered on May 01 2007

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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