Biotin Anti-CD11b + CD11c antibody [OX42] (ab33827)
![Flow Cytometry - Biotin Anti-CD11b + CD11c antibody [OX42] (ab33827)](https://www.abcam.com/ps/products/33/ab33827/Images/ab33827-47208-anti-cd11b-c-antibody-ox42-biotin-flow-cytometry.jpg "Flow Cytometry - Biotin Anti-CD11b + CD11c antibody [OX42] (ab33827)")
Key features and details
- Biotin Mouse monoclonal [OX42] to CD11b + CD11c
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: Biotin
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Biotin Anti-CD11b + CD11c antibody [OX42] -
Description
Biotin Mouse monoclonal [OX42] to CD11b + CD11c -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat CD11b. Resident peritoneal macrophages from (PVG.RT1[c] x PVG.RT1[u]) and (PVG.RT1[c] x PVG.RT1[a]) F1-hybrid rat.
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Positive control
- Rat peritoneal macrophages
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General notes
ab33827 recognises CD11b + CD11c expressed on most macrophages, including resident and activated peritoneal macrophages, Kupffer cells, around 35% of alveolar macrophages, dendritic cells, granulocytes and microglial cells in the brain.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
ab33827 recognises CD11b expressed on most macrophages, including resident and activated peritoneal macrophages, Kupffer cells, around 35% of alveolar macrophages, dendritic cells, granulocytes and microglial cells in the brain. -
Clonality
Monoclonal -
Clone number
OX42 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
Use neat - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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Flow Cyt
Use 10µl for 106 cells. Use neat - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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Cellular localization
CD11b: Membrane. CD11c: Membrane. -
Database links
Images
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Flow Cytometry - Biotin Anti-CD11b + CD11c antibody [OX42] (ab33827)FACS analysis of rat peritoneal macrophages using ab33827.
Datasheets and documents
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Datasheet download
References (5)
ab33827 has been referenced in 5 publications.
- Shi Y et al. Down Syndrome Critical Region 1 Reduces Oxidative Stress-Induced Retinal Ganglion Cells Apoptosis via CREB-Bcl-2 Pathway. Invest Ophthalmol Vis Sci 61:23 (2020). PubMed: 33104163
- Xie Y et al. Electro-acupuncture stimulation prevents remifentanil-induced postoperative hyperalgesia by suppressing spinal microglia in rats. Exp Ther Med 16:353-359 (2018). PubMed: 29896261
- Tsuruta T et al. Dental pulp-derived stem cell conditioned medium to regenerate peripheral nerves in a novel animal model of dysphagia. PLoS One 13:e0208938 (2018). PubMed: 30533035
- Uyangaa E et al. Dual TLR2/9 Recognition of Herpes Simplex Virus Infection Is Required for Recruitment and Activation of Monocytes and NK Cells and Restriction of Viral Dissemination to the Central Nervous System. Front Immunol 9:905 (2018). PubMed: 29760708
- Clements MP et al. The Wound Microenvironment Reprograms Schwann Cells to Invasive Mesenchymal-like Cells to Drive Peripheral Nerve Regeneration. Neuron 96:98-114.e7 (2017). PubMed: 28957681
