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Immunology Cell Type Markers CD Myeloid Cells
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Biotin Anti-CD13 antibody [WM15] (ab130430)

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Flow Cytometry - Biotin Anti-CD13 antibody [WM15] (ab130430)

    Key features and details

    • Biotin Mouse monoclonal [WM15] to CD13
    • Suitable for: Flow Cyt
    • Reacts with: Human, Non human primates
    • Conjugation: Biotin
    • Isotype: IgG1

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    Overview

    • Product name

      Biotin Anti-CD13 antibody [WM15]
      See all CD13 primary antibodies
    • Description

      Biotin Mouse monoclonal [WM15] to CD13
    • Host species

      Mouse
    • Conjugation

      Biotin
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human, Non human primates
    • Immunogen

      Human AML cells.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...
    • Purity

      Size exclusion
    • Clonality

      Monoclonal
    • Clone number

      WM15
    • Isotype

      IgG1
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Myeloid Cells
      • Stem Cells
      • Mesenchymal Stem Cells
      • Surface Molecules
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Dendritic Cell Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage

    Associated products

    • Alternative Versions

      • PerCP/Cy5.5® Anti-CD13 antibody [WM15] (ab157316)
      • Alexa Fluor® 488 Anti-CD13 antibody [WM15] (ab239271)
      • APC Anti-CD13 antibody [WM15] (ab239295)
      • PE/DyLight™ 594 Anti-CD13 antibody [WM15] (ab239330)
      • Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)
      • FITC Anti-CD13 antibody [WM15] (ab52461)
      • PE Anti-CD13 antibody [WM15], prediluted (ab69775)
      • Anti-CD13 antibody [WM15] (ab7417)
    • Isotype control

      • Biotin Mouse IgG1, Kappa Monoclonal [MOPC-21] - isotype control (ab18434)
    • Recombinant Protein

      • Recombinant human CD13 protein (ab134454)

    Applications

    Our Abpromise guarantee covers the use of ab130430 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use a concentration of 5 µg/ml.

    ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
    • Tissue specificity

      Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
    • Sequence similarities

      Belongs to the peptidase M1 family.
    • Domain

      Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
    • Post-translational
      modifications

      Sulfated.
      N- and O-glycosylated.
      May undergo proteolysis and give rise to a soluble form.
    • Cellular localization

      Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
    • Target information above from: UniProt accession P15144 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 290 Human
      • Omim: 151530 Human
      • SwissProt: P15144 Human
      • Unigene: 1239 Human
      • Alternative names

        • Alanyl (membrane) aminopeptidase antibody
        • Alanyl aminopeptidase antibody
        • Aminopeptidase M antibody
        • Aminopeptidase N antibody
        • AMPN_HUMAN antibody
        • ANPEP antibody
        • AP M antibody
        • AP N antibody
        • AP-M antibody
        • AP-N antibody
        • APN antibody
        • CD 13 antibody
        • CD13 antibody
        • CD13 antigen antibody
        • gp150 antibody
        • hAPN antibody
        • LAP 1 antibody
        • LAP1 antibody
        • Microsomal aminopeptidase antibody
        • Myeloid plasma membrane glycoprotein CD13 antibody
        • p150 antibody
        • PEPN antibody
        see all

      Images

      • Flow Cytometry - Biotin Anti-CD13 antibody [WM15] (ab130430)
        Flow Cytometry - Biotin Anti-CD13 antibody [WM15] (ab130430)

        Analysis by flow cytometry of ab130430 staining CD13 in human peripheral blood leukocytes.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab130430? Please let us know so that we can cite the reference in this datasheet.

      ab130430 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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