Biotin Anti-CD20 antibody [2H7] (ab123487)
Key features and details
- Biotin Mouse monoclonal [2H7] to CD20
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2b
Overview
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Product name
Biotin Anti-CD20 antibody [2H7]
See all CD20 primary antibodies -
Description
Biotin Mouse monoclonal [2H7] to CD20 -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD20. Human tonsillar B cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. -
Clonality
Monoclonal -
Clone number
2H7 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
- PE Anti-CD20 antibody [2H7] (ab112461)
- PerCP Anti-CD20 antibody [2H7] (ab114860)
- PerCP/Cy5.5® Anti-CD20 antibody [2H7] (ab157321)
- PE/DyLight™ 594 Anti-CD20 antibody [2H7] (ab187593)
- PE/Cy7® Anti-CD20 antibody [2H7] (ab234204)
- Alexa Fluor® 700 Anti-CD20 antibody [2H7] (ab269315)
- FITC Anti-CD20 antibody [2H7] (ab51792)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab123487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
This protein may be involved in the regulation of B-cell activation and proliferation. -
Tissue specificity
Expressed on B-cells. -
Involvement in disease
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
Sequence similarities
Belongs to the MS4A family. -
Post-translational
modificationsPhosphorylated. Might be functionally regulated by protein kinase(s). -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
- Unigene: 712553 Human
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Alternative names
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab123487 has been referenced in 1 publication.
- Polyak MJ & Deans JP Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure. Blood 99:3256-62 (2002). PubMed: 11964291