Anti-CD22 antibody (Biotin) (ab84227)
Key features and details
- Biotin Rabbit polyclonal to CD22
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Anti-CD22 antibody (Biotin)
See all CD22 primary antibodies -
Description
Rabbit polyclonal to CD22 (Biotin) -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
ab84227 allows the detection of at least 0.2 – 0.4 ng/well of recombinant human CD22 in ELISA assays and 1.5 - 3.0 ng/lane in Western blot, under either reducing or non-reducing conditions. -
Species reactivity
Reacts with: Human -
Immunogen
Highly pure (>98%) recombinant human CD22.
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Positive control
- Recombinant human CD22.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Lyophilized:Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Target
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Function
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. -
Tissue specificity
B-lymphocytes. -
Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
Contains 4 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
Post-translational
modificationsPhosphorylation of Tyr-762, Tyr-807 and Tyr-822 are involved in binding to SYK, GRB2 and SYK, respectively. Phosphorylation of Tyr-842 is involved in binding to SYK, PLCG2 and PIK3R1/PIK3R2.
Phosphorylated on tyrosine residues by LYN. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 933 Human
- Omim: 107266 Human
- SwissProt: P20273 Human
- Unigene: 579691 Human
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Alternative names
- B cell receptor CD22 precursor antibody
- B lymphocyte cell adhesion molecule antibody
- B-cell receptor CD22 antibody
see all
Datasheets and documents
References (0)
ab84227 has not yet been referenced specifically in any publications.
