Biotin Anti-CD3 antibody [OKT3] (ab272405)
![Flow Cytometry - Biotin Anti-CD3 antibody [OKT3] (ab272405)](https://www.abcam.com/ps/products/272/ab272405/Images/ab272405-388082-anti-cd3-antibody-okt3-biotin-flow-cytommetry-peripheral-blood-lymphocytes-human.jpg "Flow Cytometry - Biotin Anti-CD3 antibody [OKT3] (ab272405)")
Key features and details
- Biotin Mouse monoclonal [OKT3] to CD3
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Biotin Anti-CD3 antibody [OKT3]
See all CD3 primary antibodies -
Description
Biotin Mouse monoclonal [OKT3] to CD3 -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: Human peripheral blood lymphocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from TCS. Purified antibody was conjugated under optimal conditions, with unreacted biotin removed from the preparation. -
Clonality
Monoclonal -
Clone number
OKT3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Omim: 186740 Human
- Omim: 186780 Human
- Omim: 186790 Human
- Omim: 186830 Human
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Flow Cytometry - Biotin Anti-CD3 antibody [OKT3] (ab272405)
Flow cytometry analysis of human peripheral blood lymphocytes labeling CD3 with 0.5 µg ab272405 (solid line) or 0.5 µg Biotin Mouse IgG2a isotype control, followed by Streptavidin PE.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab272405 has not yet been referenced specifically in any publications.
