Biotin Anti-CD3 antibody [UCHT1] (ab191112)
![Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)](https://www.abcam.com/ps/products/191/ab191112/Images/ab191112-1-anti-Hu-CD3-Biotin-flow-cyt-peripheral-whole-blood-human.jpg "Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)")
Key features and details
- Biotin Mouse monoclonal [UCHT1] to CD3
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-CD3 antibody [UCHT1]
See all CD3 primary antibodies -
Description
Biotin Mouse monoclonal [UCHT1] to CD3 -
Host species
Mouse -
Conjugation
Biotin -
Specificity
ab191112 recognizes the CD3 antigen of the TCR/CD3 complex on mature Human T cells. The antibody reacts with the epsilon chain of the CD3 complex. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates
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Immunogen
Tissue, cells or virus corresponding to Human CD3. Human thymocytes followed by Sezary T cells.
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Positive control
- Flow Cyt: Human blood.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
ab191112 is free of unconjugated biotin. -
Clonality
Monoclonal -
Clone number
UCHT1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-CD3 antibody [UCHT1], prediluted (ab106215)
- PE/Cy5® Anti-CD3 antibody [UCHT1], prediluted (ab157300)
- PerCP/Cy5.5® Anti-CD3 antibody [UCHT1], prediluted (ab157317)
- APC Anti-CD3 antibody [UCHT1] (ab239287)
- redFluor™ 710 Anti-CD3 antibody [UCHT1] (ab241935)
- violetFluor™ 450 Anti-CD3 antibody [UCHT1] (ab241941)
- FITC Anti-CD3 antibody [UCHT1] (ab34275)
- PE/Cy7® Anti-CD3 antibody [UCHT1] (ab81992)
- APC/Cy7® Anti-CD3 antibody [UCHT1] (ab82013)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab191112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use a concentration of 1 - 12 µg/ml. |
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Omim: 186740 Human
- Omim: 186780 Human
- Omim: 186790 Human
- Omim: 186830 Human
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)Flow cytometry surface staining pattern of human peripheral whole blood stained using ab191112 (concentration in sample 0.3 µg/mL) streptavidin APC.
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![Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)](https://www.abcam.com/ps/products/191/ab191112/Images/ab191112-2-antiOPAL1-PE-flowcyt-peripheral-blood-human.jpg)
Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab191112 (concentration in sample 0.3 µg/mL) streptavidin APC.
Datasheets and documents
References (0)
ab191112 has not yet been referenced specifically in any publications.
