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  1. Link

    biotin-cd3-antibody-ucht1-ab191112.pdf

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Immunology Adaptive Immunity T Cells CD
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Biotin Anti-CD3 antibody [UCHT1] (ab191112)

  • Datasheet
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Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)
  • Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)

Key features and details

  • Biotin Mouse monoclonal [UCHT1] to CD3
  • Suitable for: Flow Cyt (Intra), Flow Cyt
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG1

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Overview

  • Product name

    Biotin Anti-CD3 antibody [UCHT1]
    See all CD3 primary antibodies
  • Description

    Biotin Mouse monoclonal [UCHT1] to CD3
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Specificity

    ab191112 recognizes the CD3 antigen of the TCR/CD3 complex on mature Human T cells. The antibody reacts with the epsilon chain of the CD3 complex.
  • Tested applications

    Suitable for: Flow Cyt (Intra), Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Non human primates
  • Immunogen

    Tissue, cells or virus corresponding to Human CD3. Human thymocytes followed by Sezary T cells.

  • Positive control

    • Flow Cytometry: human peripheral whole blood. Flow Cyt (Intra): Human blood.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.0975% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    ab191112 is free of unconjugated biotin.
  • Clonality

    Monoclonal
  • Clone number

    UCHT1
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Signal Transduction
    • Antibodies
    • cd3

Associated products

  • Alternative Versions

    • PerCP Anti-CD3 antibody [UCHT1], prediluted (ab106215)
    • PE/Cy5® Anti-CD3 antibody [UCHT1], prediluted (ab157300)
    • PerCP/Cy5.5® Anti-CD3 antibody [UCHT1], prediluted (ab157317)
    • APC Anti-CD3 antibody [UCHT1] (ab239287)
    • redFluor™ 710 Anti-CD3 antibody [UCHT1] (ab241935)
    • violetFluor™ 450 Anti-CD3 antibody [UCHT1] (ab241941)
    • FITC Anti-CD3 antibody [UCHT1] (ab34275)
    • PE/Cy7® Anti-CD3 antibody [UCHT1] (ab81992)
    • APC/Cy7® Anti-CD3 antibody [UCHT1] (ab82013)
  • Isotype control

    • Biotin Mouse IgG1, Kappa Monoclonal [MOPC-21] - isotype control (ab18434)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab191112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use a concentration of 1 - 12 µg/ml.
Flow Cyt
Use a concentration of 1 - 12 µg/ml.
Notes
Flow Cyt (Intra)
Use a concentration of 1 - 12 µg/ml.
Flow Cyt
Use a concentration of 1 - 12 µg/ml.

Target

  • Function

    The CD3 complex mediates signal transduction.
  • Involvement in disease

    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similarities

    Contains 1 ITAM domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P04234 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 915 Human
    • Entrez Gene: 916 Human
    • Entrez Gene: 917 Human
    • Entrez Gene: 919 Human
    • Omim: 186740 Human
    • Omim: 186780 Human
    • Omim: 186790 Human
    • Omim: 186830 Human
    • SwissProt: P04234 Human
    • SwissProt: P07766 Human
    • SwissProt: P09693 Human
    • SwissProt: P20963 Human
    • Unigene: 504048 Human
    see all
  • Alternative names

    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • Cd247 antibody
    • CD247 antigen antibody
    • CD247 antigen, zeta subunit antibody
    • CD247 molecule antibody
    • CD3 antibody
    • CD3 antigen, delta subunit antibody
    • CD3 delta antibody
    • CD3 epsilon antibody
    • CD3 eta antibody
    • CD3 gamma antibody
    • CD3 molecule delta polypeptide antibody
    • CD3 molecule, epsilon polypeptide antibody
    • CD3 molecule, gamma polypeptide antibody
    • CD3 zeta antibody
    • CD3-DELTA antibody
    • CD3d antibody
    • CD3D antigen delta polypeptide antibody
    • CD3d antigen, delta polypeptide (TiT3 complex) antibody
    • CD3d molecule delta antibody
    • CD3d molecule delta CD3 TCR complex antibody
    • CD3d molecule, delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • CD3E antibody
    • CD3e antigen antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule epsilon CD3 TCR complex antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3epsilon antibody
    • CD3G antibody
    • CD3g antigen antibody
    • CD3G antigen gamma polypeptide antibody
    • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
    • CD3g molecule gamma antibody
    • CD3g molecule gamma CD3 TCR complex antibody
    • CD3g molecule, gamma (CD3-TCR complex) antibody
    • CD3H antibody
    • CD3Q antibody
    • CD3Z antibody
    • CD3zeta antibody
    • Ctg3 antibody
    • FLJ17620 antibody
    • FLJ17664 antibody
    • FLJ18683 antibody
    • FLJ79544 antibody
    • FLJ94613 antibody
    • IMD19 antibody
    • Leu-4 antibody
    • MGC138597 antibody
    • OKT3, delta chain antibody
    • OTTHUMP00000032544 antibody
    • T cell receptor antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor T3 gamma chain antibody
    • T cell receptor T3 zeta chain antibody
    • T cell receptor zeta chain antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T cell surface glycoprotein CD3 gamma chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, delta subunit of T3 antibody
    • T-cell antigen receptor complex, epsilon subunit of T3 antibody
    • T-cell antigen receptor complex, gamma subunit of T3 antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3 antibody
    • T3d antibody
    • T3e antibody
    • T3g antibody
    • T3z antibody
    • TCRE antibody
    • TCRk antibody
    • Tcrz antibody
    • TCRzeta antibody
    see all

Images

  • Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)
    Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)
    Flow cytometry surface staining pattern of human peripheral whole blood stained using ab191112 (concentration in sample 0.3 µg/mL) streptavidin APC.
  • Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)
    Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)
    Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab191112 (concentration in sample 0.3 µg/mL) streptavidin APC.

Protocols

  • Flow cytometry protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab191112? Please let us know so that we can cite the reference in this datasheet.

ab191112 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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