Biotin Anti-CD3 antibody [UCHT1] (ab191112)
![Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)](https://www.abcam.com/ps/products/191/ab191112/Images/ab191112-1-anti-Hu-CD3-Biotin-flow-cyt-peripheral-whole-blood-human.jpg "Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)")
Key features and details
- Biotin Mouse monoclonal [UCHT1] to CD3
- Suitable for: Flow Cyt (Intra), Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-CD3 antibody [UCHT1]
See all CD3 primary antibodies -
Description
Biotin Mouse monoclonal [UCHT1] to CD3 -
Host species
Mouse -
Conjugation
Biotin -
Specificity
ab191112 recognizes the CD3 antigen of the TCR/CD3 complex on mature Human T cells. The antibody reacts with the epsilon chain of the CD3 complex. -
Tested applications
Suitable for: Flow Cyt (Intra), Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Non human primates
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Immunogen
Tissue, cells or virus corresponding to Human CD3. Human thymocytes followed by Sezary T cells.
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Positive control
- Flow Cytometry: human peripheral whole blood. Flow Cyt (Intra): Human blood.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
ab191112 is free of unconjugated biotin. -
Clonality
Monoclonal -
Clone number
UCHT1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- PerCP Anti-CD3 antibody [UCHT1], prediluted (ab106215)
- PE/Cy5® Anti-CD3 antibody [UCHT1], prediluted (ab157300)
- PerCP/Cy5.5® Anti-CD3 antibody [UCHT1], prediluted (ab157317)
- APC Anti-CD3 antibody [UCHT1] (ab239287)
- redFluor™ 710 Anti-CD3 antibody [UCHT1] (ab241935)
- violetFluor™ 450 Anti-CD3 antibody [UCHT1] (ab241941)
- FITC Anti-CD3 antibody [UCHT1] (ab34275)
- PE/Cy7® Anti-CD3 antibody [UCHT1] (ab81992)
- APC/Cy7® Anti-CD3 antibody [UCHT1] (ab82013)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 1 - 12 µg/ml.
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| Flow Cyt |
Use a concentration of 1 - 12 µg/ml.
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| Notes |
|---|
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Flow Cyt (Intra)
Use a concentration of 1 - 12 µg/ml. |
|
Flow Cyt
Use a concentration of 1 - 12 µg/ml. |
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Omim: 186740 Human
- Omim: 186780 Human
- Omim: 186790 Human
- Omim: 186830 Human
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Images
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Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)Flow cytometry surface staining pattern of human peripheral whole blood stained using ab191112 (concentration in sample 0.3 µg/mL) streptavidin APC.
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![Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)](https://www.abcam.com/ps/products/191/ab191112/Images/ab191112-2-antiOPAL1-PE-flowcyt-peripheral-blood-human.jpg)
Flow Cytometry - Biotin Anti-CD3 antibody [UCHT1] (ab191112)Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab191112 (concentration in sample 0.3 µg/mL) streptavidin APC.
Datasheets and documents
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Datasheet download
References (0)
ab191112 has not yet been referenced specifically in any publications.
