Key features and details
- Biotin Mouse monoclonal [UCHT1] to CD3
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Product nameBiotin Anti-CD3 antibody [UCHT1]
See all CD3 primary antibodies
DescriptionBiotin Mouse monoclonal [UCHT1] to CD3
Specificityab191112 recognizes the CD3 antigen of the TCR/CD3 complex on mature Human T cells. The antibody reacts with the epsilon chain of the CD3 complex.
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Non human primates
Tissue, cells or virus corresponding to Human CD3. Human thymocytes followed by Sezary T cells.
- Flow Cyt: Human blood.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Purification notesab191112 is free of unconjugated biotin.
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Our Abpromise guarantee covers the use of ab191112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 1 - 12 µg/ml.|
FunctionThe CD3 complex mediates signal transduction.
Involvement in diseaseDefects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Sequence similaritiesContains 1 ITAM domain.
- Information by UniProt
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
Flow cytometry surface staining pattern of human peripheral whole blood stained using ab191112 (concentration in sample 0.3 µg/mL) streptavidin APC.
Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab191112 (concentration in sample 0.3 µg/mL) streptavidin APC.
ab191112 has not yet been referenced specifically in any publications.