Biotin Anti-CD3 epsilon antibody [C363.29B (YCD3-1)] (ab25688)
Key features and details
- Biotin Rat monoclonal [C363.29B (YCD3-1)] to CD3 epsilon
- Suitable for: IP, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2b
Overview
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Product name
Biotin Anti-CD3 epsilon antibody [C363.29B (YCD3-1)]
See all CD3 epsilon primary antibodies -
Description
Biotin Rat monoclonal [C363.29B (YCD3-1)] to CD3 epsilon -
Host species
Rat -
Conjugation
Biotin -
Specificity
In the presence of Fc receptor bearing accessory cells, this antibody can activate primed and naïve T cell in vitro. When immobilized it can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. -
Tested applications
Suitable for: IP, Flow Cyt, Functional Studiesmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to CD3 epsilon. IL-4 producing Th2 cell lines including D10
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Epitope
This antibody recognises an epitope on the 25kDa epsilon chain of the CD3/TCR complex. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
C363.29B (YCD3-1) -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use 3µg for 106 cells.
ab18542 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| Functional Studies |
Use at an assay dependent concentration.
In vitro depletion of CD3+ cells & In vitro activation of T cells (accessory cells must be provided). |
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
|
Flow Cyt
Use 3µg for 106 cells. ab18542 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
Functional Studies
Use at an assay dependent concentration. In vitro depletion of CD3+ cells & In vitro activation of T cells (accessory cells must be provided). |
Target
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Function
The CD3 complex mediates signal transduction. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12501 Mouse
- SwissProt: P22646 Mouse
- Unigene: 210361 Mouse
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Alternative names
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab25688 has not yet been referenced specifically in any publications.