Biotin Anti-CD32B + CD32A antibody [AT10] (ab30355)
![Flow Cytometry - Biotin Anti-CD32B + CD32A antibody [AT10] (ab30355)](https://www.abcam.com/ps/products/30/ab30355/Images/ab30355-40336-anti-cd32-antibody-at10-biotin-flow-cytometry.jpg "Flow Cytometry - Biotin Anti-CD32B + CD32A antibody [AT10] (ab30355)")
Key features and details
- Biotin Mouse monoclonal [AT10] to CD32B + CD32A
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Biotin Anti-CD32B + CD32A antibody [AT10] -
Description
Biotin Mouse monoclonal [AT10] to CD32B + CD32A -
Host species
Mouse -
Conjugation
Biotin -
Specificity
According to the CD32A + CD32B Cluster workshop (Leucocyte Typing V, pp823, pp828), clone AT10 reacts equally well with both Fc gamma RII a and b-expressing cells.
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Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD32A. K562 cell line
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Positive control
- Human peripheral blood monocytes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AT10 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab30355 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 10µl for 106 cells.
ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
|
Flow Cyt
Use 10µl for 106 cells. ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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Target
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Cellular localization
CD32B: Cell membrane. CD32A: Cell membrane. -
Database links
- Entrez Gene: 2212 Human
- Entrez Gene: 2213 Human
- Omim: 146790 Human
- Omim: 604590 Human
- SwissProt: P12318 Human
- SwissProt: P31994 Human
- Unigene: 352642 Human
- Unigene: 654395 Human
see all
Images
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Flow Cytometry - Biotin Anti-CD32B + CD32A antibody [AT10] (ab30355)
Flow cytometric analysis of human peripheral blood monocytes with 10µl for 106 cells of anti human CD32A + CD32B Biotin conjugated (ab30355).
Datasheets and documents
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Datasheet download
References (4)
ab30355 has been referenced in 4 publications.
- Tzeng SJ et al. Fc?RIIB mediates antigen-independent inhibition on human B lymphocytes through Btk and p38 MAPK. J Biomed Sci 22:87 (2015). PubMed: 26475492
- Lilliehöök I et al. Expression of adhesion and Fcgamma-receptors on canine blood eosinophils and neutrophils studied by anti-human monoclonal antibodies. Vet Immunol Immunopathol 61:181-93 (1998). PubMed: 9613433
- Metes D et al. Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene. Blood 91:2369-80 (1998). PubMed: 9516136
- Greenman J et al. Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors. Mol Immunol 28:1243-54 (1991). PubMed: 1835758
