Biotin Anti-CD4 antibody [MEM-241] (ab28069)

Submit a review Q&A (1)References (1)

Key features and details

  • Biotin Mouse monoclonal [MEM-241] to CD4
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG1

Overview

  • Product name

    Biotin Anti-CD4 antibody [MEM-241]
    See all CD4 primary antibodies

  • Description

    Biotin Mouse monoclonal [MEM-241] to CD4

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    CD4 fusion protein (Human).

  • General notes

    The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab28069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
Flow Cyt
Use at an assay dependent concentration.

ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

  • Cellular localization

    Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 4 antibody
      • CD4 (L3T4) antibody
      • CD4 antibody
      • CD4 antigen (p55) antibody
      • CD4 antigen antibody
      • CD4 molecule antibody
      • CD4 receptor antibody
      • CD4+ Lymphocyte deficiency, included antibody
      • CD4_HUMAN antibody
      • CD4mut antibody
      • L3T4 antibody
      • Leu3 antibody
      • Ly-4 antibody
      • Lymphocyte antigen CD4 antibody
      • MGC165891 antibody
      • OTTHUMP00000238897 antibody
      • p55 antibody
      • T cell antigen T4 antibody
      • T cell antigen T4/LEU3 antibody
      • T cell differentiation antigen L3T4 antibody
      • T cell OKT4 deficiency, included antibody
      • T cell surface antigen T4/Leu 3 antibody
      • T cell surface antigen T4/Leu3 antibody
      • T cell surface glycoprotein CD4 antibody
      • T-cell surface antigen T4/Leu-3 antibody
      • T-cell surface glycoprotein CD4 antibody
      • W3/25 antibody
      • W3/25 antigen antibody
      see all

    References (1)

    Publishing research using ab28069? Please let us know so that we can cite the reference in this datasheet.

    ab28069 has been referenced in 1 publication.

    • Chen YT  et al. Quantitatively Resolving Ligand-Receptor Bonds on Cell Surfaces Using Force-Induced Remnant Magnetization Spectroscopy. ACS Cent Sci 2:75-9 (2016). PubMed: 27163031

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Needa biotinylated CD4 antibody to use in a sandwich with ab51312.

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    Answer

    Thanks for your call today and for your questions about our CD4 antibodies. Since neither ab28069 or ab34271 has been tested in a sandwich ELISA with ab51312, I can offer a discount off a future purchase if you buy one of these biotinylated CD4 antibodies now, test it in sandwich ELISA with ab28069 or ab34271, and submit feedback to us in the form of an Abreview. The discount would be worth of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test one of these antibodies. I will then send a discount code. 2. Purchase ab28069 or ab34271 either by phone, fax, or online (www.abcam.com). 3. Test it in sandwich ELISA with ab51312. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the sandwich does not work, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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