Biotin Anti-CD43 antibody [MEM-59] (ab28104)

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Key features and details

  • Biotin Mouse monoclonal [MEM-59] to CD43
  • Suitable for: IHC-P, IP, Flow Cyt, WB
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 700 APC/Cy7® FITC Unconjugated

Overview

  • Product name

    Biotin Anti-CD43 antibody [MEM-59]
    See all CD43 primary antibodies

  • Description

    Biotin Mouse monoclonal [MEM-59] to CD43

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Specificity

    The antibody recognizes neuraminidase sensitive epitope on CD43 antigen (Leukosialin), which is involved in lymphocyte activation.

  • Tested applications

    Suitable for: IHC-P, IP, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to Human CD43. Human T lymphocytes

  • General notes

    The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab28104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration.

Target

  • Function

    One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.

  • Tissue specificity

    Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

  • Post-translational
    modifications

    Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 43 antibody
      • CD43 antibody
      • CD43 antigen antibody
      • Galactoglycoprotein antibody
      • GALGP antibody
      • GPL 115 antibody
      • GPL115 antibody
      • Human gene for sialophorin antibody
      • Leucocyte sialoglycoprotein antibody
      • LEUK_HUMAN antibody
      • Leukocyte large sialoglycoprotein antibody
      • Leukocyte sialoglycoprotein antibody
      • Leukosialin antibody
      • LSN antibody
      • Ly-48 antibody
      • sialophorin (gpL115, leukosialin, CD43) antibody
      • Sialophorin antibody
      • Spn antibody
      see all

    References (1)

    Publishing research using ab28104? Please let us know so that we can cite the reference in this datasheet.

    ab28104 has been referenced in 1 publication.

    • Alvarado M  et al. MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation. Eur J Immunol 25:1051-5 (1995). PubMed: 7537669

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