Biotin Anti-CD45RA antibody [MEM-56] (ab28109)
![Flow Cytometry - Biotin Anti-CD45RA antibody [MEM-56] (ab28109)](https://www.abcam.com/ps/products/28/ab28109/Images/ab28109-279884-anti-cd45ra-antibody-mem-56-biotin-flow-cytometry.jpg "Flow Cytometry - Biotin Anti-CD45RA antibody [MEM-56] (ab28109)")
Key features and details
- Biotin Mouse monoclonal [MEM-56] to CD45RA
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2b
Related conjugates and formulations
Overview
-
Product name
Biotin Anti-CD45RA antibody [MEM-56]
See all CD45RA primary antibodies -
Description
Biotin Mouse monoclonal [MEM-56] to CD45RA -
Host species
Mouse -
Conjugation
Biotin -
Specificity
ab28109 recognises CD45RA antigen. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD45RA. Human thymocytes and T lymphocytes
Database link: P08575-8 -
General notes
The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.0
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-56 -
Isotype
IgG2b -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
Flow Cyt
Use a concentration of 1 - 5 µg/ml. ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. -
Involvement in disease
Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
Multiple sclerosis -
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
Domain
The first PTPase domain interacts with SKAP1. -
Post-translational
modificationsHeavily N- and O-glycosylated. -
Cellular localization
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
-
Database links
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
-
Alternative names
- B220 antibody
- CD45 antibody
- CD45 antigen antibody
see all
Images
-
Flow Cytometry - Biotin Anti-CD45RA antibody [MEM-56] (ab28109)Surface staining of human peripheral blood with anti-CD45RA (MEM-56) biotin / streptavidin-APC.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab28109 has been referenced in 1 publication.
- Bazil V et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). PubMed: 2534480