Biotin Anti-CD59 antibody [MEM-43] (ab28114)
![Flow Cytometry - Biotin Anti-CD59 antibody [MEM-43] (ab28114)](https://www.abcam.com/ps/products/28/ab28114/Images/ab28114-278851-anti-cd59-antibody-mem-43-biotin-flow-cytometry.jpg "Flow Cytometry - Biotin Anti-CD59 antibody [MEM-43] (ab28114)")
Key features and details
- Biotin Mouse monoclonal [MEM-43] to CD59
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-CD59 antibody [MEM-43]
See all CD59 primary antibodies -
Description
Biotin Mouse monoclonal [MEM-43] to CD59 -
Host species
Mouse -
Conjugation
Biotin -
Specificity
ab28114 recognises CD59 antigen. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue/ cell preparation (Human): Thymocytes and T lymphocytes.
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Epitope
ab28114 reacts with a well defined epitope (W40, R-53) on CD59 molecule. -
Positive control
- Flow Cyt: human HL-60 cells
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General notes
The antibody is conjugated with Biotin-LC-NHS and it is free of unconjugated biotin.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-43 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1 - 2 µg/ml.
ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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Flow Cyt
Use a concentration of 1 - 2 µg/ml. ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. -
Involvement in disease
Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300]. -
Sequence similarities
Contains 1 UPAR/Ly6 domain. -
Post-translational
modificationsN- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes. -
Cellular localization
Cell membrane. Secreted. Soluble form found in a number of tissues. - Information by UniProt
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Database links
- Entrez Gene: 966 Human
- Omim: 107271 Human
- SwissProt: P13987 Human
- Unigene: 278573 Human
- Unigene: 709466 Human
- Unigene: 710641 Human
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Alternative names
- 16.3A5 antibody
- 1F5 antibody
- 1F5 antigen antibody
see all
Images
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Flow Cytometry - Biotin Anti-CD59 antibody [MEM-43] (ab28114)
Flow Cytometry analysis of positive HL60 cells (human promyelocytic leukemia), and negative SP2 (murine myeloma) cells labeling CD59 with ab28114.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab28114 has been referenced in 3 publications.
- Cebecauer M et al. Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells. Biochem Biophys Res Commun 243:706-10 (1998). PubMed: 9500981
- Stefanová I et al. GPI-anchored cell-surface molecules complexed to protein tyrosine kinases. Science 254:1016-9 (1991). PubMed: 1719635
- Stefanová I et al. Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol 26:153-61 (1989). PubMed: 2918859