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    biotin-cd59-antibody-mem-43-ab28114.pdf

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Immunology Cell Type Markers CD Non-lineage
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Biotin Anti-CD59 antibody [MEM-43] (ab28114)

  • Datasheet
  • SDS
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Flow Cytometry - Biotin Anti-CD59 antibody [MEM-43] (ab28114)

    Key features and details

    • Biotin Mouse monoclonal [MEM-43] to CD59
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: Biotin
    • Isotype: IgG2a

    You may also be interested in

    Protein
    Product image
    Recombinant Human CD59 protein (ab134887)

    View more associated products

    Overview

    • Product name

      Biotin Anti-CD59 antibody [MEM-43]
      See all CD59 primary antibodies
    • Description

      Biotin Mouse monoclonal [MEM-43] to CD59
    • Host species

      Mouse
    • Conjugation

      Biotin
    • Specificity

      ab28114 recognises CD59 antigen.
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue/ cell preparation (Human): Thymocytes and T lymphocytes.

    • Epitope

      ab28114 reacts with a well defined epitope (W40, R-53) on CD59 molecule.
    • General notes

      The antibody is conjugated with Biotin-LC-NHS and it is free of unconjugated biotin.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      MEM-43
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Non-lineage
      • Immunology
      • Innate Immunity
      • Complement
      • MAC
      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Kinases
      • Other
      • Stem Cells
      • Hematopoietic Progenitors
      • Surface Molecules
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Innate and adaptive immunity

    Associated products

    • Alternative Versions

      • FITC Anti-CD59 antibody [MEM-43], prediluted (ab18237)
      • Alexa Fluor® 647 Anti-CD59 antibody [MEM-43], prediluted (ab187769)
      • PE Anti-CD59 antibody [MEM-43] (ab36421)
      • APC Anti-CD59 antibody [MEM-43] (ab36467)
      • Anti-CD59 antibody [MEM-43] (ab9182)
    • Isotype control

      • Biotin Mouse IgG2a, kappa [MOPC-173] - Isotype Control (ab18454)
    • Recombinant Protein

      • Recombinant Human CD59 protein (ab134887)

    Applications

    Our Abpromise guarantee covers the use of ab28114 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt 1/40.

    ab97679 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
      The soluble form from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes.
    • Involvement in disease

      Defects in CD59 are the cause of CD59 deficiency (CD59D) [MIM:612300].
    • Sequence similarities

      Contains 1 UPAR/Ly6 domain.
    • Post-translational
      modifications

      N- and O-glycosylated. The N-glycosylation mainly consists of a family of biantennary complex-type structures with and without lactosamine extensions and outer arm fucose residues. Also significant amounts of triantennary complexes (22%). Variable sialylation also present in the Asn-43 oligosaccharide. The predominant O-glycans are mono-sialylated forms of the disaccharide, Gal-beta-1,3GalNAc, and their sites of attachment are probably on Thr-76 and Thr-77. The GPI-anchor of soluble urinary CD59 has no inositol-associated phospholipid, but is composed of seven different GPI-anchor variants of one or more monosaccharide units. Major variants contain sialic acid, mannose and glucosamine Sialic acid linked to an N-acetylhexosamine-galactose arm is present in two variants.
      Glycated. Glycation is found in diabetic subjects, but only at minimal levels in nondiabetic subjects. Glycated CD59 lacks MAC-inhibitory function and confers to vascular complications of diabetes.
    • Cellular localization

      Cell membrane. Secreted. Soluble form found in a number of tissues.
    • Target information above from: UniProt accession P13987 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 966 Human
      • Omim: 107271 Human
      • SwissProt: P13987 Human
      • Unigene: 278573 Human
      • Unigene: 709466 Human
      • Unigene: 710641 Human
      • Alternative names

        • 16.3A5 antibody
        • 1F5 antibody
        • 1F5 antigen antibody
        • 20 kDa homologous restriction factor antibody
        • CD 59 antibody
        • CD_antigen=CD59 antibody
        • CD59 antibody
        • CD59 antigen antibody
        • CD59 antigen complement regulatory protein antibody
        • CD59 antigen p18 20 antibody
        • CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344) antibody
        • CD59 glycoprotein antibody
        • CD59 molecule antibody
        • CD59 molecule complement regulatory protein antibody
        • CD59_HUMAN antibody
        • Cd59a antibody
        • Complement regulatory protein antibody
        • EJ16 antibody
        • EJ30 antibody
        • EL32 antibody
        • FLJ38134 antibody
        • FLJ92039 antibody
        • G344 antibody
        • HRF 20 antibody
        • HRF-20 antibody
        • HRF20 antibody
        • Human leukocyte antigen MIC11 antibody
        • Ly 6 like protein antibody
        • Lymphocytic antigen CD59/MEM43 antibody
        • MAC inhibitory protein antibody
        • MAC IP antibody
        • MAC-inhibitory protein antibody
        • MAC-IP antibody
        • MACIF antibody
        • MACIP antibody
        • MEM43 antibody
        • MEM43 antigen antibody
        • Membrane attack complex (MAC) inhibition factor antibody
        • Membrane attack complex inhibition factor antibody
        • Membrane inhibitor of reactive lysis antibody
        • MGC2354 antibody
        • MIC11 antibody
        • MIN1 antibody
        • MIN2 antibody
        • MIN3 antibody
        • MIRL antibody
        • MSK21 antibody
        • p18 20 antibody
        • Protectin antibody
        • Surface antigen recognized by monoclonal antibody 16.3A5 antibody
        • T cell activating protein antibody
        see all

      Images

      • Flow Cytometry - Biotin Anti-CD59 antibody [MEM-43] (ab28114)
        Flow Cytometry - Biotin Anti-CD59 antibody [MEM-43] (ab28114)
        Flow Cytometry analysis of HL60 and SP2 cells labeling CD59 with Anti-CD59 antibody [MEM-43] (Biotin) (ab28114).

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (3)

      Publishing research using ab28114? Please let us know so that we can cite the reference in this datasheet.

      ab28114 has been referenced in 3 publications.

      • Cebecauer M  et al. Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells. Biochem Biophys Res Commun 243:706-10 (1998). PubMed: 9500981
      • Stefanová I  et al. GPI-anchored cell-surface molecules complexed to protein tyrosine kinases. Science 254:1016-9 (1991). PubMed: 1719635
      • Stefanová I  et al. Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol. Mol Immunol 26:153-61 (1989). PubMed: 2918859

      Customer reviews and Q&As

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      There are currently no Customer reviews or Questions for ab28114.
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