Key features and details
- Biotin Mouse monoclonal [MEM-259] to CD63
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Product nameBiotin Anti-CD63 antibody [MEM-259]
See all CD63 primary antibodies
DescriptionBiotin Mouse monoclonal [MEM-259] to CD63
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Human
Tissue, cells or virus corresponding to Human CD63.
- Activated platelets, neutrophils and basophils. Human spleen tissue.
The antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab134331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
FunctionThis antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
Tissue specificityDysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
Cellular localizationCell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
- Information by UniProt
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained usingab134331 (concentration in sample 0.6 μg/mL, Streptavidin APC).
Flow cytometry multicolor surface staining of IgE stimulated peripheral blood mononuclear cells stained usingab134331 (concentration in sample 0.6 μg/mL, Streptavidin APC) and anti-human CD203c PE antibody (20 μL reagent / 100 μL of peripheral whole blood).
Flow Cytometry analysis of (surface staining) of IgE stimulated human peripheral whole blood using ab134331 (concentration in sample 0.6 μg/mL, Streptavidin APC). CD63 positive CD203c positive basophil granulocytes (red-filled) from neutrophil granulocytes (black-dashed).
ab134331 has been referenced in 2 publications.
- Neo SY et al. CD73 immune checkpoint defines regulatory NK cells within the tumor microenvironment. J Clin Invest 130:1185-1198 (2020). PubMed: 31770109
- Huang L et al. A dual-signal amplification platform for sensitive fluorescence biosensing of leukemia-derived exosomes. Nanoscale 10:20289-20295 (2018). PubMed: 30371719