Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)

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Flow Cytometry - Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)

    Key features and details

    • Biotin Rat monoclonal [53-6.7] to CD8 alpha
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: Biotin
    • Isotype: IgG2a

    Overview

    • Product name

      Biotin Anti-CD8 alpha antibody [53-6.7]
      See all CD8 alpha primary antibodies

    • Description

      Biotin Rat monoclonal [53-6.7] to CD8 alpha

    • Host species

      Rat

    • Conjugation

      Biotin

    • Specificity

      ab25176 recognises a chain of the murine CD8 heterodimer, Mr 32-34 kDa.

    • Tested applications

      Suitable for: Flow Cytmore details

    • Species reactivity

      Reacts with: Mouse

    • Immunogen

      The details of the immunogen for this antibody are not available.

    • General notes


      In vivo and in vitro treatment with ab25176 effectively depletes CD8 alpha+ cells. ab25176 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Avoid freeze / thaw cycle. Do Not Freeze.

    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.16% Sodium phosphate, 0.87% Sodium chloride
    • Concentration information loading...

    • Purity

      Affinity purified

    • Primary antibody notes

      In vivo and in vitro treatment with ab25176 effectively depletes CD8 alpha+ cells. ab25176 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

    • Clonality

      Monoclonal

    • Clone number

      53-6.7

    • Isotype

      IgG2a

    • Light chain type

      kappa

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab25176 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use 2µl for 106 cells.

    ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     
    Notes
    Flow Cyt
    Use 2µl for 106 cells.

    ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

    • Involvement in disease

      Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Post-translational
      modifications

      All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.

    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • alpha polypeptide (p32) antibody
        • CD_antigen=CD8a antibody
        • CD8 antibody
        • CD8 antigen alpha polypeptide antibody
        • CD8 antigen alpha polypeptide (p32) antibody
        • CD8 antigen, alpha polypeptide (p32) antibody
        • CD8a antibody
        • CD8A antigen antibody
        • CD8A molecule antibody
        • CD8A_HUMAN antibody
        • Leu2 antibody
        • Leu2 T lymphocyte antigen antibody
        • Ly 2 antibody
        • Ly 35 antibody
        • Ly B antibody
        • Ly2 antibody
        • Ly3 antibody
        • Ly35 antibody
        • LyB antibody
        • Lyt 2.1 lymphocyte differentiation antigen (AA at 100) antibody
        • LYT3 antibody
        • MAL antibody
        • OKT8 T cell antigen antibody
        • OTTHUMP00000160760 antibody
        • OTTHUMP00000160764 antibody
        • OTTHUMP00000203528 antibody
        • OTTHUMP00000203721 antibody
        • p32 antibody
        • T cell antigen Leu2 antibody
        • T cell co receptor antibody
        • T lymphocyte differentiation antigen T8/Leu 2 antibody
        • T-cell surface glycoprotein CD8 alpha chain antibody
        • T-cell surface glycoprotein Lyt 2 antibody
        • T-lymphocyte differentiation antigen T8/Leu-2 antibody
        • T8 T cell antigen antibody
        • T8/Leu-2 T-lymphocyte differentiation antigen antibody
        see all

      Images

      • Flow Cytometry - Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)
        Flow Cytometry - Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)

         

         

        Flow cytometry analysis showing C57Bl/6 splenocytes stained with ab25176 (solid line). Isotype control antibody was Rat Ig2a Biotin (dashed line).

      References (0)

      Publishing research using ab25176? Please let us know so that we can cite the reference in this datasheet.

      ab25176 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Using the antibody with drosophila expressed mouse CD8 alpha.
      The ab gives multiple bands

      Read More
      Answer

      Thank you for contacting us.
      I have received following reply from my colleague when asked for immunogen;
      "The immunogen used to produce clone 53-6.7 was mouse thymus or spleen. Generation of this clone is described in the below reference article. At this time, we do not have any information regarding cross reactivity with Drosophila proteins. We apologize for any inconvenience.

      Ledbetter, J. A. and L. A. Herzenberg. 1979. Immunol. Rev. 47:63."
      Assuming that the immunogen is from CD8 alpha chain, I blasted it and found no homology with any of Drosophila protein. So I am sorry I will not be able to provide you any definite answer in this case.
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

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