Biotin Anti-CD8 antibody [MEM-31] (ab28090)

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Flow Cytometry - Biotin Anti-CD8 antibody [MEM-31] (ab28090)
  • Flow Cytometry - Biotin Anti-CD8 antibody [MEM-31] (ab28090)

Key features and details

  • Biotin Mouse monoclonal [MEM-31] to CD8
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG2a

Overview

  • Product name

    Biotin Anti-CD8 antibody [MEM-31]
    See all CD8 primary antibodies

  • Description

    Biotin Mouse monoclonal [MEM-31] to CD8

  • Host species

    Mouse

  • Conjugation

    Biotin

  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus corresponding to Human CD8. Crude thymus membrane fraction

  • Epitope

    ab28090 recognizes a conformationally dependent epitope of CD8 antigen (does not react with formaldehyde-fixed cells)

  • General notes

    The purified antibody is conjugated with Biotin-LC-NHS.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab28090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use 1µg for 106 cells.

ab18454 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
Notes
Flow Cyt
Use 1µg for 106 cells.

ab18454 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 
Application notes
Is unsuitable for WB.

Target

  • Function

    Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

  • Involvement in disease

    Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Post-translational
    modifications

    All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Form

      CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.

    • Alternative names

      • CD8 antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8a antigen antibody
      • CD8a molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      see all

    Images

    • Flow Cytometry - Biotin Anti-CD8 antibody [MEM-31] (ab28090)
      Flow Cytometry - Biotin Anti-CD8 antibody [MEM-31] (ab28090)

      Flow cytometry analysis (surface staining) of human peripheral blood using ab28090.

    • Flow Cytometry - Biotin Anti-CD8 antibody [MEM-31] (ab28090)
      Flow Cytometry - Biotin Anti-CD8 antibody [MEM-31] (ab28090)
      Overlay histogram showing peripheral blood lymphocytes stained with ab28090 (red line). The cells were incubated with the antibody (ab28090, 1µg/1x106 cells) followed by Streptavidin-FITC conjugated protein for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) with secondary antibody DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

    References (1)

    Publishing research using ab28090? Please let us know so that we can cite the reference in this datasheet.

    ab28090 has been referenced in 1 publication.

    • Horejsí V  et al. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) 32:12-25 (1986). PubMed: 2422063

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