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Immunology Adaptive Immunity T Cells CD
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Biotin Anti-CD82 antibody [C33] (ab190292)

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Flow Cytometry - Biotin Anti-CD82 antibody [C33] (ab190292)

    Key features and details

    • Biotin Mouse monoclonal [C33] to CD82
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: Biotin
    • Isotype: IgG2a

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    Overview

    • Product name

      Biotin Anti-CD82 antibody [C33]
      See all CD82 primary antibodies
    • Description

      Biotin Mouse monoclonal [C33] to CD82
    • Host species

      Mouse
    • Conjugation

      Biotin
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Tissue, cells or virus corresponding to Human CD82. C91/PL (Human HTLV-1+ T cell line).

    • Positive control

      • Flow Cyt: Human peripheral blood cells.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...
    • Purity

      Size exclusion
    • Purification notes

      Purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
    • Clonality

      Monoclonal
    • Clone number

      C33
    • Isotype

      IgG2a
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Immunology
      • Cell Type Markers
      • CD
      • NK Cells
      • Immunology
      • Cell Type Markers
      • CD
      • Platelets
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Tumor Suppressors
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • Other

    Associated products

    • Alternative Versions

      • Anti-CD82 antibody [C33] (ab140238)
      • FITC Anti-CD82 antibody [C33], prediluted (ab176514)
    • Isotype control

      • Biotin Mouse IgG2a, kappa [MOPC-173] - Isotype Control (ab18454)
    • Recombinant Protein

      • Recombinant Human CD82 protein (ab114340)
    • Related Products

      • Recombinant Human CD82 protein (ab114340)

    Applications

    Our Abpromise guarantee covers the use of ab190292 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use a concentration of 1 - 12 µg/ml.

    Target

    • Function

      Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
    • Tissue specificity

      Lymphoid specific.
    • Sequence similarities

      Belongs to the tetraspanin (TM4SF) family.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession P27701 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3732 Human
      • Omim: 600623 Human
      • SwissProt: P27701 Human
      • Unigene: 527778 Human
      • Alternative names

        • 4F9 antibody
        • Antigen detected by monoclonal and antibody IA4 antibody
        • C33 antibody
        • C33 antigen antibody
        • CD 82 antibody
        • CD82 antibody
        • CD82 antigen antibody
        • CD82 molecule antibody
        • CD82_HUMAN antibody
        • GR 15 antibody
        • GR15 antibody
        • IA 4 antibody
        • IA4 antibody
        • Inducible membrane protein antibody
        • Inducible membrane protein R2 antibody
        • KAI 1 antibody
        • KAI1 antibody
        • Kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4)) antibody
        • Kangai 1 antibody
        • Kangai1 antibody
        • Leukocyte surface antigen R2 antibody
        • Metastasis suppressor Kangai 1 antibody
        • Metastasis suppressor Kangai-1 antibody
        • Metastasis suppressor Kangai1 antibody
        • Prostate cancer antimetastasis gene KAI1 antibody
        • R2 antibody
        • R2 leukocyte antigen antibody
        • SAR 2 antibody
        • SAR2 antibody
        • ST 6 antibody
        • ST6 antibody
        • Suppression of tumorigenicity 6 antibody
        • Suppression of tumorigenicity 6 prostate antibody
        • Suppressor of tumorigenicity 6 antibody
        • Suppressor of tumorigenicity 6 protein antibody
        • Tetraspanin 27 antibody
        • Tetraspanin-27 antibody
        • Tetraspanin27 antibody
        • Tspan 27 antibody
        • Tspan-27 antibody
        • Tspan27 antibody
        see all

      Images

      • Flow Cytometry - Biotin Anti-CD82 antibody [C33] (ab190292)
        Flow Cytometry - Biotin Anti-CD82 antibody [C33] (ab190292)
        Flow cytometry analysis of mouse splenocytes labeling CD82 epsilon using ab190292 followed by a Streptavidin-PE comjugate. Surface staining.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab190292? Please let us know so that we can cite the reference in this datasheet.

      ab190292 has not yet been referenced specifically in any publications.

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