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  1. Link

    biotin-cd9-antibody-mem-61-ab28094.pdf

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Cardiovascular Blood Platelets
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Biotin Anti-CD9 antibody [MEM-61] (ab28094)

  • Datasheet
  • SDS
Reviews (1) Submit a question

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Key features and details

  • Biotin Mouse monoclonal [MEM-61] to CD9
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 APC APC/Cy7® FITC Unconjugated

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Overview

  • Product name

    Biotin Anti-CD9 antibody [MEM-61]
    See all CD9 primary antibodies
  • Description

    Biotin Mouse monoclonal [MEM-61] to CD9
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD9. Pre B cell line NALM-6

  • Epitope

    The antibody recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a protein of 24 kDa belonging to tetraspans family (TM4SF).
  • General notes

    The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    MEM-61
  • Isotype

    IgG1
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Blood
    • Other
    • Immunology
    • Cell Type Markers
    • CD
    • Platelets
    • Stem Cells
    • Embryonic Stem Cells
    • Surface Molecules
    • Developmental Biology
    • Reproduction
    • Fertilisation
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Intracellular

Associated products

  • Alternative Versions

    • FITC Anti-CD9 antibody [MEM-61] (ab18241)
    • Alexa Fluor® 647 Anti-CD9 antibody [MEM-61] (ab187776)
    • Anti-CD9 antibody [MEM-61] (ab2215)
    • APC/Cy7® Anti-CD9 antibody [MEM-61] (ab239306)
    • APC Anti-CD9 antibody [MEM-61], prediluted (ab82389)
  • Isotype control

    • Biotin Mouse IgG1, Kappa Monoclonal [MOPC-21] - isotype control (ab18434)
  • Recombinant Protein

    • Recombinant Human CD9 protein (Tagged) (ab152262)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab28094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Notes
Flow Cyt
Use at an assay dependent concentration.

ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
  • Tissue specificity

    Expressed by a variety of hematopoietic and epithelial cells.
  • Sequence similarities

    Belongs to the tetraspanin (TM4SF) family.
  • Post-translational
    modifications

    Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P21926 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 928 Human
    • Omim: 143030 Human
    • SwissProt: P21926 Human
    • Unigene: 114286 Human
    • Alternative names

      • Tetraspanin 29 antibody
      • 5H9 antibody
      • 5H9 antigen antibody
      • Antigen defined by monoclonal antibody 602 29 antibody
      • Antigen defined by monoclonal antibody 60229 antibody
      • BA-2/p24 antigen antibody
      • BA2 antibody
      • BTCC 1 antibody
      • BTCC1 antibody
      • CD9 antibody
      • CD9 antigen antibody
      • CD9 antigen p24 antibody
      • CD9 molecule antibody
      • CD9_HUMAN antibody
      • Cell growth inhibiting gene 2 protein antibody
      • Cell growth-inhibiting gene 2 protein antibody
      • DRAP 27 antibody
      • DRAP27 antibody
      • GIG2 antibody
      • Growth inhibiting gene 2 protein antibody
      • Leukocyte antigen MIC3 antibody
      • MIC3 antibody
      • Motility related protein antibody
      • Motility-related protein antibody
      • MRP 1 antibody
      • MRP-1 antibody
      • MRP1 antibody
      • p24 antibody
      • p24 antigen antibody
      • Tetraspanin-29 antibody
      • Tspan 29 antibody
      • Tspan-29 antibody
      • TSPAN29 antibody
      see all

    Protocols

    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab28094? Please let us know so that we can cite the reference in this datasheet.

    ab28094 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    ELISA abreview for Biotin Anti-CD9 antibody [MEM-61]

    Good
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Human Cell (extracellular vesicles BT-474)
    Specification
    extracellular vesicles BT-474
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
    Type
    Sandwich (Detection)
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 30 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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