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Biotin Anti-CD90 / Thy1 antibody [5E10] (ab134361)

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Flow Cytometry - Biotin Anti-CD90 / Thy1 antibody [5E10] (ab134361)

    Key features and details

    • Biotin Mouse monoclonal [5E10] to CD90 / Thy1
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Conjugation: Biotin
    • Isotype: IgG1

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    Overview

    • Product name

      Biotin Anti-CD90 / Thy1 antibody [5E10]
      See all CD90 / Thy1 primary antibodies
    • Description

      Biotin Mouse monoclonal [5E10] to CD90 / Thy1
    • Host species

      Mouse
    • Conjugation

      Biotin
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      HEL Human erythroleukemia cell line

    • Positive control

      • Human erythroleukemia (HEL) cells
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. Do Not Freeze. Store In the Dark.
    • Storage buffer

      pH: 7.20
      Preservative: 0.09% Sodium azide
      Constituents: 0.1% Gelatin, 99% PBS
    • Concentration information loading...
    • Purity

      Protein G purified
    • Clonality

      Monoclonal
    • Clone number

      5E10
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Adaptive Immunity
      • T Cells
      • CD
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • B Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Lymphoid
      • T Lymphocytic Lineage
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Monocytic Lineage

    Associated products

    • Alternative Versions

      • Anti-CD90 / Thy1 antibody [5E10] (ab123511)
      • FITC Anti-CD90 / Thy1 antibody [5E10] (ab124527)
      • PerCP/Cy5.5® Anti-CD90 / Thy1 antibody [5E10] (ab134360)
      • APC Anti-CD90 / Thy1 antibody [5E10] (ab139364)
      • PE/Cy5® Anti-CD90 / Thy1 antibody [5E10] (ab95698)
      • PE Anti-CD90 / Thy1 antibody [5E10] (ab95700)
    • Isotype control

      • Biotin Mouse IgG1, Kappa Monoclonal [MOPC-21] - isotype control (ab18434)
    • Recombinant Protein

      • Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)

    Applications

    Our Abpromise guarantee covers the use of ab134361 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt Use 1µg for 105-8 cells.

    (In a final volume of 100 µl.)

     

     

     

    ab18434 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
    • Sequence similarities

      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell membrane.
    • Target information above from: UniProt accession P04216 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7070 Human
      • Omim: 188230 Human
      • SwissProt: P04216 Human
      • Unigene: 644697 Human
      • Alternative names

        • CD7 antibody
        • CD90 antibody
        • CD90 antigen antibody
        • CDw90 antibody
        • FLJ33325 antibody
        • MGC128895 antibody
        • T25 antibody
        • Theta antigen antibody
        • Thy 1 antibody
        • Thy 1 cell surface antigen antibody
        • Thy 1 membrane glycoprotein antibody
        • Thy 1 T cell antigen antibody
        • Thy 1.2 antibody
        • Thy-1 antigen antibody
        • Thy-1 membrane glycoprotein antibody
        • Thy1 antibody
        • Thy1 antigen antibody
        • Thy1 T cell antigen antibody
        • Thy1.1 antibody
        • Thy1.2 antibody
        • THY1_HUMAN antibody
        • Thymus cell antigen 1, theta antibody
        see all

      Images

      • Flow Cytometry - Biotin Anti-CD90 / Thy1 antibody [5E10] (ab134361)
        Flow Cytometry - Biotin Anti-CD90 / Thy1 antibody [5E10] (ab134361)
        Flow cytometry analysis of Human erythroleukemia (HEL) cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Biotin (open histogram) or 0.5 µg ab134361 (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab134361? Please let us know so that we can cite the reference in this datasheet.

      ab134361 has been referenced in 1 publication.

      • Billaud M  et al. Classification and Functional Characterization of Vasa Vasorum-Associated Perivascular Progenitor Cells in Human Aorta. Stem Cell Reports 9:292-303 (2017). PubMed: 28552602

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