Biotin Anti-CLIP170 antibody (ab240434)
Key features and details
- Biotin Goat polyclonal to CLIP170
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Biotin Anti-CLIP170 antibody
See all CLIP170 primary antibodies -
Description
Biotin Goat polyclonal to CLIP170 -
Host species
Goat -
Conjugation
Biotin -
Specificity
This antibody is expected to recognize both reported isoforms (NP_002947.1; NP_937883.1). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human CLIP170 aa 1404-1417 (Cysteine residue).
Sequence:C-PHSTHHGSRGEERP
Database link: P30622 -
Positive control
- WB: Human breast cancer tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab240434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 162 kDa. 1 hour primary incubation is recommended for this product. |
Target
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Function
Seems to be a intermediate filament associated protein that links endocytic vesicles to microtubules. -
Tissue specificity
Highly expressed in the Reed-Sternberg cells of Hodgkin disease. -
Sequence similarities
Contains 2 CAP-Gly domains. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton. Localizes to the tips of microtubules. - Information by UniProt
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Database links
- Entrez Gene: 6249 Human
- Entrez Gene: 56430 Mouse
- Entrez Gene: 65201 Rat
- Omim: 179838 Human
- SwissProt: P30622 Human
- SwissProt: Q922J3 Mouse
- Unigene: 524809 Human
- Unigene: 241109 Mouse
see all -
Alternative names
- CAP GLY domain containing linker protein 1 antibody
- CAP-Gly domain-containing linker protein 1 antibody
- CLIP 170 antibody
see all
Images
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Western blot - Biotin Anti-CLIP170 antibody (ab240434)Biotin Anti-CLIP170 antibody (ab240434) at 1 µg/ml + Human breast cancer tissue in RIPA buffer at 35 µg
Secondary
Streptavidin-HRP
Predicted band size: 162 kDaPrimary incubation for 1 hour.
Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab240434 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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