Product nameBiotin conjugation kit (with inbuilt quantification)
Biotin conjugation kit (with inbuilt quantification) (ab272214) is a unique antibody/protein conjugation system with inbuilt biotin quantification, which obviates the need for a separate determination of incorporated biotin using the HABA assay.
The conjugation procedure is extremely simple: the antibody to be labeled is used to reconstitute a freeze-dried activator compound, and the resulting mixture is added to the biotin chromophore ligand vial. After a fixed time, the conjugate is desalted to remove excess ligand and the absorbance of the conjugate is read at A350 and A280. These absorbance values allow you to determine the yield of conjugate, its concentration, and the average number of biotins per antibody.
Storage instructionsPlease refer to protocols.
Components 3 x 100 µg Activator 3 vials Biotin ligand 3 vials Column equilibration buffer 1 unit DMSO 1 vial NAP-5 Column 3 units Quencher reagent 3 units Reaction Buffer 1 vial
ab272214 has not yet been referenced specifically in any publications.