Biotin Anti-CX3CR1 antibody [2A9-1] (ab95620)
![Flow Cytometry - Biotin Anti-CX3CR1 antibody [2A9-1] (ab95620)](https://www.abcam.com/ps/products/95/ab95620/Images/ab95620-109892-anti-cx3cr1-antibody-2a9-1-biotin-flow-cytometry.jpg "Flow Cytometry - Biotin Anti-CX3CR1 antibody [2A9-1] (ab95620)")
Key features and details
- Biotin Rat monoclonal [2A9-1] to CX3CR1
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2b
Overview
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Product name
Biotin Anti-CX3CR1 antibody [2A9-1]
See all CX3CR1 primary antibodies -
Description
Biotin Rat monoclonal [2A9-1] to CX3CR1 -
Host species
Rat -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
CX3CR1-EGFP fusion protein.
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Positive control
- Normal Human peripheral blood cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2A9-1 -
Isotype
IgG2b -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab95620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.25µl for 105-8 cells. Use a final volume of 100µl.
ab18542 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1. -
Tissue specificity
Expressed in lymphoid and neural tissues. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1524 Human
- Omim: 601470 Human
- SwissProt: P49238 Human
- Unigene: 78913 Human
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Alternative names
- Beta chemokine receptor-like 1 antibody
- C X3 C CKR 1 antibody
- C-X3-C CKR-1 antibody
see all
Images
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Flow Cytometry - Biotin Anti-CX3CR1 antibody [2A9-1] (ab95620)Staining of normal Human peripheral blood cells with 0.125µg/100µl rat IgG1 isotype control Biotin (left) or 0.125µg/100µl ab95620 (right), followed by Streptavidin PE.
Samples were subsequently stained intracellularly with an anti Human Perforin FITC. Cells were gated on CD16+CD56+ using anti-CD16 eFluor 450 and anti-CD56 APC antibodies.
Datasheets and documents
References (1)
ab95620 has been referenced in 1 publication.
- Sun C et al. ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma. Int J Oncol 57:249-263 (2020). PubMed: 32319605
