Key features and details
- Biotin Rabbit polyclonal to CXCL2
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
Product nameBiotin Anti-CXCL2 antibody
See all CXCL2 primary antibodies
DescriptionBiotin Rabbit polyclonal to CXCL2
Tested applicationsSuitable for: WB, Sandwich ELISAmore details
Species reactivityReacts with: Recombinant fragment
Predicted to work with: Rat
- WB: Recombinant rat CXCL2 protein. sELISA: Recombinant rat CXCL2 protein.
FormLyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage bufferConstituent: PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab245861 in the following tested applications.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.|
|Sandwich ELISA||Use a concentration of 0.25 - 1 µg/ml.|
FunctionProduced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsThe N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells.
- Information by UniProt
- C-X-C motif chemokine 2 antibody
- Chemokine (C X C motif) ligand 2 antibody
- Chemokine, CXC motif, ligand 2 antibody
To detect rat CXCL2 by Western Blot analysis ab245861 can be used at a concentration of 0.1 - 0.2 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant rat CXCL2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant rat CXCL2, respectively.
To detect rat CXCL2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab245861 is required.
This biotinylated polyclonal antibody, in conjunction with a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant rat CXCL2.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245861 has not yet been referenced specifically in any publications.