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  1. Link

    biotin-cxcl5-antibody-ab186670.pdf

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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)
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Biotin Anti-CXCL5 antibody (ab186670)

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ELISA - Biotin Anti-CXCL5 antibody (ab186670)
  • Sandwich ELISA - Biotin Anti-CXCL5 antibody (ab186670)

Key features and details

  • Biotin Rabbit polyclonal to CXCL5
  • Suitable for: Sandwich ELISA, ELISA
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG

You may also be interested in

Protein
Recombinant Mouse CXCL5 protein (ab50173)

View more associated products

Overview

  • Product name

    Biotin Anti-CXCL5 antibody
    See all CXCL5 primary antibodies
  • Description

    Biotin Rabbit polyclonal to CXCL5
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: Sandwich ELISA, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CXCL5 aa 5-78.
    Sequence:

    SSRAAR VPGPSSSLCA LLVLLLLLTQ PGPIASAGPA AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKV


    Database link: P42830
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Recombinant Human CXCL5 protein.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    ab186670 was produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant Human CXCL5 . ab186670 was purified by affinity chromatography and then biotinylated.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Chemokines

Associated products

  • Isotype control

    • Biotin Rabbit IgG - Isotype Control (ab200208)
  • Recombinant Protein

    • Recombinant Mouse CXCL5 protein (ab50173)

Applications

Our Abpromise guarantee covers the use of ab186670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • Function

    Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P42830 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6374 Human
    • Omim: 600324 Human
    • SwissProt: P42830 Human
    • Unigene: 89714 Human
    • Alternative names

      • AMCFII antibody
      • C-X-C motif chemokine 5 antibody
      • C-X-C motif chemokine ligand 5 antibody
      • Chemokine (C X C motif) ligand 5 antibody
      • chemokine (C-X-C motif) ligand 5 antibody
      • Cxcl5 antibody
      • CXCL5_HUMAN antibody
      • ENA 78 antibody
      • ENA-78 (8-78) antibody
      • ENA-78(1-78) antibody
      • ENA-78(9-78) antibody
      • ENA78 antibody
      • Epithelial derived neutrophil activating protein 78 antibody
      • Epithelial-derived neutrophil-activating protein 78 antibody
      • Lipopolysaccharide-induced CXC chemokine antibody
      • Neutrophil activating peptide ENA 78 antibody
      • Neutrophil activating protein 78 antibody
      • Neutrophil-activating peptide ENA-78 antibody
      • neutrophil-activating protein 78 antibody
      • SCYB5 antibody
      • Small inducible cytokine B5 antibody
      • small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78) antibody
      • small inducible cytokine subfamily B, member 5 antibody
      • Small-inducible cytokine B5 antibody
      see all

    Images

    • ELISA - Biotin Anti-CXCL5 antibody (ab186670)
      ELISA - Biotin Anti-CXCL5 antibody (ab186670)

      Direct  ELISA analysis of recombinant Human CXCL5 using ab186670 at a concentration of 0.25-1.0 μg/ml.
      ab186670, used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human CXCL5.

    • Sandwich ELISA - Biotin Anti-CXCL5 antibody (ab186670)
      Sandwich ELISA - Biotin Anti-CXCL5 antibody (ab186670)

      Sandwich ELISA analysis of recombinant Human CXCL5 using ab186670 at a concentration of 0.25-1.0 μg/ml.

      ab186670, used in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human CXCL5.

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab186670? Please let us know so that we can cite the reference in this datasheet.

    ab186670 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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