Biotin Anti-Cytokeratin 18 antibody [C-04] (ab27553)

Reviews (2)Q&A (1)References (4)
Western blot - Biotin Anti-Cytokeratin 18 antibody [C-04] (ab27553)

    Key features and details

    • Biotin Mouse monoclonal [C-04] to Cytokeratin 18
    • Suitable for: WB
    • Reacts with: Human
    • Conjugation: Biotin
    • Isotype: IgG1

    Overview

    • Product name

      Biotin Anti-Cytokeratin 18 antibody [C-04]
      See all Cytokeratin 18 primary antibodies

    • Description

      Biotin Mouse monoclonal [C-04] to Cytokeratin 18

    • Host species

      Mouse

    • Conjugation

      Biotin

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mammals

    • Immunogen

      Tissue, cells or virus corresponding to Human Cytokeratin 18. Cytoskeleton preparation of epidermal carcinoma cell line A431

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab27553 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500. Predicted molecular weight: 47 kDa.
    Notes
    WB
    1/500. Predicted molecular weight: 47 kDa.

    Target

    • Function

      Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

    • Tissue specificity

      Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

    • Involvement in disease

      Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].

    • Sequence similarities

      Belongs to the intermediate filament family.

    • Post-translational
      modifications

      Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
      Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
      O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.

    • Cellular localization

      Cytoplasm > perinuclear region.
    • Information by UniProt

    • Database links

      • Alternative names

        • Cell proliferation inducing gene 46 protein antibody
        • Cell proliferation inducing protein 46 antibody
        • Cell proliferation-inducing gene 46 protein antibody
        • CK 18 antibody
        • CK-18 antibody
        • CK18 antibody
        • CYK 18 antibody
        • CYK18 antibody
        • Cytokeratin 18 antibody
        • Cytokeratin endo B antibody
        • Cytokeratin-18 antibody
        • K 18 antibody
        • K18 antibody
        • K1C18_HUMAN antibody
        • KA18 antibody
        • Keratin 18 antibody
        • Keratin 18, type I antibody
        • Keratin D antibody
        • keratin, type I cytoskeletal 18 antibody
        • Keratin-18 antibody
        • Krt18 antibody
        see all

      Images

      • Western blot - Biotin Anti-Cytokeratin 18 antibody [C-04] (ab27553)
        Western blot - Biotin Anti-Cytokeratin 18 antibody [C-04] (ab27553)
        Western blotting analysis of cytokeratin 18 in HeLa cells with anti-cytokeratin 18 (C-04) biotin.

      References (4)

      Publishing research using ab27553? Please let us know so that we can cite the reference in this datasheet.

      ab27553 has been referenced in 4 publications.

      • Gao Q  et al. Paeoniflorin prevents postoperative peritoneal adhesion formation in an experimental rat model. Oncotarget 8:93899-93911 (2017). PubMed: 29212197
      • Prater MD  et al. Mammary stem cells have myoepithelial cell properties. Nat Cell Biol 16:942-50 (2014). IHC-P ; Mouse . PubMed: 25173976
      • Kim S  et al. FGFR2 promotes breast tumorigenicity through maintenance of breast tumor-initiating cells. PLoS One 8:e51671 (2013). IHC-P ; Mouse . PubMed: 23300950
      • Drobinskaya I  et al. Scalable selection of hepatocyte- and hepatocyte precursor-like cells from culture of differentiating transgenically modified murine embryonic stem cells. Stem Cells 26:2245-56 (2008). ICC/IF . PubMed: 18556507

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Question

      Does you anti-cyteokeratin-18 antibody #ab27553 react with rat and mouse species? Are they recognized linearly with human species?   Thank you,  

      Read More
      Answer

      Thank you for contacting us.

      This product has been tested in mouse and because of a 97% homology with rat and an 87% homolgy with human it is predicted to work in both species. There is an eight amino acid gap near the N terminus between the mouse and human proteins/

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 18 antibody [C-04] (Biotin)

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (Mammary gland)
      Specification
      Mammary gland
      Fixative
      Acetone:methanol (1:1)
      Permeabilization
      Yes - 0.025% Triton X
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 30 2010

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cytokeratin 18 antibody [C-04] (Biotin)

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (mouse ES cell drived hepatocyte-like cells)
      Specification
      mouse ES cell drived hepatocyte-like cells
      Fixative
      Formaldehyde
      Permeabilization
      Yes - Triton X-100
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Dr. Eugen Kolossov

      Verified customer

      Submitted Nov 01 2007

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