Biotin Anti-Cytokeratin 18 antibody [C-04] (ab27553)
![Western blot - Biotin Anti-Cytokeratin 18 antibody [C-04] (ab27553)](https://www.abcam.com/ps/products/27/ab27553/Images/ab27553-473344-biotin-anti-cytokeratin-18-antibody-c04-western-blot-ht29.jpg "Western blot - Biotin Anti-Cytokeratin 18 antibody [C-04] (ab27553)")
Key features and details
- Biotin Mouse monoclonal [C-04] to Cytokeratin 18
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Biotin Anti-Cytokeratin 18 antibody [C-04]
See all Cytokeratin 18 primary antibodies -
Description
Biotin Mouse monoclonal [C-04] to Cytokeratin 18 -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mammals
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Immunogen
Tissue, cells or virus corresponding to Human Cytokeratin 18. Cytoskeleton preparation of epidermal carcinoma cell line A431
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Positive control
- WB: HT-29 (reducing and non-reducing conditions) cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
C-04 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500. Predicted molecular weight: 47 kDa.
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| Notes |
|---|
|
WB
1/500. Predicted molecular weight: 47 kDa. |
Target
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Function
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
Tissue specificity
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
Involvement in disease
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
Cellular localization
Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
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Alternative names
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
Images
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Western blot - Biotin Anti-Cytokeratin 18 antibody [C-04] (ab27553)All lanes : Biotin Anti-Cytokeratin 18 antibody [C-04] (ab27553) at 2 µg/ml
Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (reducing conditions)
Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (reducing conditions)
Lane 3 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (non-reducing conditions)
Lane 4 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (non-reducing conditions)
Secondary
All lanes : Conjugated Streptavidin
Predicted band size: 47 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?Proteolytic fragment observed at approximately 25 kDa.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab27553 has been referenced in 4 publications.
- Gao Q et al. Paeoniflorin prevents postoperative peritoneal adhesion formation in an experimental rat model. Oncotarget 8:93899-93911 (2017). PubMed: 29212197
- Prater MD et al. Mammary stem cells have myoepithelial cell properties. Nat Cell Biol 16:942-50 (2014). IHC-P ; Mouse . PubMed: 25173976
- Kim S et al. FGFR2 promotes breast tumorigenicity through maintenance of breast tumor-initiating cells. PLoS One 8:e51671 (2013). IHC-P ; Mouse . PubMed: 23300950
- Drobinskaya I et al. Scalable selection of hepatocyte- and hepatocyte precursor-like cells from culture of differentiating transgenically modified murine embryonic stem cells. Stem Cells 26:2245-56 (2008). ICC/IF . PubMed: 18556507