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  1. Link

    biotin-cytokeratin-18-antibody-da-7-ab27549.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)

  • Datasheet
  • SDS
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Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)

    Key features and details

    • Biotin Mouse monoclonal [DA-7] to Cytokeratin 18
    • Suitable for: WB
    • Reacts with: Human
    • Conjugation: Biotin
    • Isotype: IgG1

    Overview

    • Product name

      Biotin Anti-Cytokeratin 18 antibody [DA-7]
      See all Cytokeratin 18 primary antibodies
    • Description

      Biotin Mouse monoclonal [DA-7] to Cytokeratin 18
    • Host species

      Mouse
    • Conjugation

      Biotin
    • Specificity

      ab27549 recognises Cytokeratin peptide 18. We have data to indicate that this antibody may not cross react with wide range of other species. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Human breast carcinoma cell line PMC-42.

    • Positive control

      • Western blot and immunocytochemistry: HeLa human cervix carcinoma cell line, MCF-7 human breast adenocarcinoma cell line. Immunohistochemistry: epithelial tissue (skin). ELISA: MCF-7 human breast adenocarcinoma cell line
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      DA-7
    • Isotype

      IgG1
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Signal Transduction
      • Cytoskeleton / ECM
      • Cytoskeleton
      • Intermediate Filaments
      • Class I
      • Cytokeratins

    Associated products

    • Alternative Versions

      • Anti-Cytokeratin 18 antibody [DA-7] (ab669)
    • Isotype control

      • Biotin Mouse IgG1, Kappa Monoclonal [MOPC-21] - isotype control (ab18434)

    Applications

    Our Abpromise guarantee covers the use of ab27549 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500. Predicted molecular weight: 45 kDa.

    Target

    • Function

      Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
    • Tissue specificity

      Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
    • Involvement in disease

      Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
    • Sequence similarities

      Belongs to the intermediate filament family.
    • Post-translational
      modifications

      Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
      Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
      O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
    • Cellular localization

      Cytoplasm > perinuclear region.
    • Target information above from: UniProt accession P05783 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3875 Human
      • Omim: 148070 Human
      • SwissProt: P05783 Human
      • Unigene: 406013 Human
      • Alternative names

        • Cell proliferation inducing gene 46 protein antibody
        • Cell proliferation inducing protein 46 antibody
        • Cell proliferation-inducing gene 46 protein antibody
        • CK 18 antibody
        • CK-18 antibody
        • CK18 antibody
        • CYK 18 antibody
        • CYK18 antibody
        • Cytokeratin 18 antibody
        • Cytokeratin endo B antibody
        • Cytokeratin-18 antibody
        • K 18 antibody
        • K18 antibody
        • K1C18_HUMAN antibody
        • KA18 antibody
        • Keratin 18 antibody
        • Keratin 18, type I antibody
        • Keratin D antibody
        • keratin, type I cytoskeletal 18 antibody
        • Keratin-18 antibody
        • Krt18 antibody
        see all

      Images

      • Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)
        Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)
        Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549) at 1 µg/ml + HeLa (50 million) cells re-suspended in 1 ml cold Lysis buffer.

        Performed under non-reducing conditions.

        Predicted band size: 45 kDa

      Protocols

      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab27549? Please let us know so that we can cite the reference in this datasheet.

      ab27549 has been referenced in 1 publication.

      • Sweeney E  et al. Quantitative multiplexed quantum dot immunohistochemistry. Biochem Biophys Res Commun 374:181-6 (2008). ICC ; Human . PubMed: 18621021

      Customer reviews and Q&As

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      Please use the links above to contact us or submit feedback about this product.

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