Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)
![Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)](https://www.abcam.com/ps/products/27/ab27549/Images/ab27549-473346-biotin-anti-cytokeratin-18-antibody-da7-western-blot-human.jpg "Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)")
Key features and details
- Biotin Mouse monoclonal [DA-7] to Cytokeratin 18
- Suitable for: WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Biotin Anti-Cytokeratin 18 antibody [DA-7]
See all Cytokeratin 18 primary antibodies -
Description
Biotin Mouse monoclonal [DA-7] to Cytokeratin 18 -
Host species
Mouse -
Conjugation
Biotin -
Specificity
ab27549 recognises Cytokeratin peptide 18. We have data to indicate that this antibody may not cross react with wide range of other species. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Cytokeratin 18. Human breast carcinoma cell line PMC-42
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Positive control
- WB: HT-29 (reducing and non-reducing conditions) whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
DA-7 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa.
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| Notes |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 45 kDa. |
Target
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Function
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
Tissue specificity
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
Involvement in disease
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
Cellular localization
Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
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Alternative names
- Cell proliferation inducing gene 46 protein antibody
- Cell proliferation inducing protein 46 antibody
- Cell proliferation-inducing gene 46 protein antibody
see all
Images
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Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)All lanes : Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549) at 2 µg/ml
Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (reducing conditions)
Lane 2 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (reducing conditions)
Lane 3 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate (non-reducing conditions)
Lane 4 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate (non-reducing conditions)
Predicted band size: 45 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab27549 has been referenced in 1 publication.
- Sweeney E et al. Quantitative multiplexed quantum dot immunohistochemistry. Biochem Biophys Res Commun 374:181-6 (2008). ICC ; Human . PubMed: 18621021