Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)

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Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)

    Key features and details

    • Biotin Mouse monoclonal [DA-7] to Cytokeratin 18
    • Suitable for: WB
    • Reacts with: Human
    • Conjugation: Biotin
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Unconjugated

    Overview

    • Product name

      Biotin Anti-Cytokeratin 18 antibody [DA-7]
      See all Cytokeratin 18 primary antibodies

    • Description

      Biotin Mouse monoclonal [DA-7] to Cytokeratin 18

    • Host species

      Mouse

    • Conjugation

      Biotin

    • Specificity

      ab27549 recognises Cytokeratin peptide 18. We have data to indicate that this antibody may not cross react with wide range of other species. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Tissue, cells or virus corresponding to Human Cytokeratin 18. Human breast carcinoma cell line PMC-42

    • Positive control

      • Western blot and immunocytochemistry: HeLa human cervix carcinoma cell line, MCF-7 human breast adenocarcinoma cell line. Immunohistochemistry: epithelial tissue (skin). ELISA: MCF-7 human breast adenocarcinoma cell line

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab27549 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/500. Predicted molecular weight: 45 kDa.
    Notes
    WB
    1/500. Predicted molecular weight: 45 kDa.

    Target

    • Function

      Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

    • Tissue specificity

      Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.

    • Involvement in disease

      Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].

    • Sequence similarities

      Belongs to the intermediate filament family.

    • Post-translational
      modifications

      Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
      Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
      O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.

    • Cellular localization

      Cytoplasm > perinuclear region.
    • Information by UniProt

    • Database links

      • Alternative names

        • Cell proliferation inducing gene 46 protein antibody
        • Cell proliferation inducing protein 46 antibody
        • Cell proliferation-inducing gene 46 protein antibody
        • CK 18 antibody
        • CK-18 antibody
        • CK18 antibody
        • CYK 18 antibody
        • CYK18 antibody
        • Cytokeratin 18 antibody
        • Cytokeratin endo B antibody
        • Cytokeratin-18 antibody
        • K 18 antibody
        • K18 antibody
        • K1C18_HUMAN antibody
        • KA18 antibody
        • Keratin 18 antibody
        • Keratin 18, type I antibody
        • Keratin D antibody
        • keratin, type I cytoskeletal 18 antibody
        • Keratin-18 antibody
        • Krt18 antibody
        see all

      Images

      • Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)
        Western blot - Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549)
        Biotin Anti-Cytokeratin 18 antibody [DA-7] (ab27549) at 1 µg/ml + HeLa (50 million) cells re-suspended in 1 ml cold Lysis buffer.

        Performed under non-reducing conditions.

        Predicted band size: 45 kDa

      References (1)

      Publishing research using ab27549? Please let us know so that we can cite the reference in this datasheet.

      ab27549 has been referenced in 1 publication.

      • Sweeney E  et al. Quantitative multiplexed quantum dot immunohistochemistry. Biochem Biophys Res Commun 374:181-6 (2008). ICC ; Human . PubMed: 18621021

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