Biotin Anti-Cytokeratin 19 antibody [A53-B/A2] (ab239265)
![Flow Cytometry - Biotin Anti-Cytokeratin 19 antibody [A53-B/A2] (ab239265)](https://www.abcam.com/ps/products/239/ab239265/Images/ab239265-318737-239265444.jpg "Flow Cytometry - Biotin Anti-Cytokeratin 19 antibody [A53-B/A2] (ab239265)")
Key features and details
- Biotin Mouse monoclonal [A53-B/A2] to Cytokeratin 19
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-Cytokeratin 19 antibody [A53-B/A2]
See all Cytokeratin 19 primary antibodies -
Description
Biotin Mouse monoclonal [A53-B/A2] to Cytokeratin 19 -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Cytokeratin 19. (MCF7 human breast adenocarcinoma cell line).
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Positive control
- Flow Cyt: MCF7 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.0975% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
Purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin. -
Clonality
Monoclonal -
Clone number
A53-B/A2 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab239265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | 1/500. |
Target
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Function
Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. -
Tissue specificity
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin. -
Sequence similarities
Belongs to the intermediate filament family. -
Developmental stage
Present in hair follicles at all stages of development. -
Domain
This keratin differs from all other IF proteins in lacking the C-terminal tail domain. - Information by UniProt
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Database links
- Entrez Gene: 3880 Human
- Omim: 148020 Human
- SwissProt: P08727 Human
- Unigene: 654568 Human
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Alternative names
- 40 kDa keratin intermediate filament antibody
- CK 19 antibody
- CK-19 antibody
see all
Images
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Flow Cytometry - Biotin Anti-Cytokeratin 19 antibody [A53-B/A2] (ab239265)
Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cell line labeling Cytokeratin 19 with ab239265 at 1/500 dilution (red), compared with a negative control. Intracellular staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab239265 has not yet been referenced specifically in any publications.
