Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832)
![Western blot - Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832)](https://www.abcam.com/ps/products/173/ab173832/Images/ab173832-197524-anti-ddddk-tag-antibody-fg4r-equivalent-to-flag-antibodies-from-sigma-biotin-western-blot.jpg "Western blot - Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832)")
Key features and details
- Biotin Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: WB, ICC/IF, IP
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG2b
Overview
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Product name
Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R]
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
Biotin Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin.
Sequence:DYKDDDDK
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Positive control
- E. coli lysate containing a multi-epitope tagged protein.
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General notes
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
FG4R -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| ICC/IF |
1/200.
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| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
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WB
Use at an assay dependent concentration. |
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ICC/IF
1/200. |
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IP
Use at an assay dependent concentration. |
Target
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Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
Alternative names
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
Images
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Western blot - Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832)All lanes : Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg
Secondary
All lanes : Streptavidin-HRP at 1/20000 dilution
Developed using the ECL technique.Note: Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Incubation with ab173832 was for 1 hour at room temperature. Incubation with secondary antibody was for 30 miutes at room temperature.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab173832 has not yet been referenced specifically in any publications.