Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832)
![Western blot - Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832)](https://www.abcam.com/ps/products/173/ab173832/Images/ab173832-197524-anti-ddddk-tag-antibody-fg4r-equivalent-to-flag-antibodies-from-sigma-biotin-western-blot.jpg "Western blot - Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832)")
Key features and details
- Biotin Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence)
- Suitable for: WB, ICC/IF, IP
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG2b
Overview
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Product name
Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R]
See all DDDDK tag (Binds to FLAG® tag sequence) primary antibodies -
Description
Biotin Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence) -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence) conjugated to keyhole limpet haemocyanin.
Sequence:DYKDDDDK
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Positive control
- E. coli lysate containing a multi-epitope tagged protein.
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General notes
FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
FG4R -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab173832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. | |
| ICC/IF | 1/200. | |
| IP | Use at an assay dependent concentration. |
Target
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Relevance
This is a useful tool for the localisation and characterisation of DDDDK tagged proteins (Binds to FLAG® tag sequence). -
Alternative names
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
see all
Images
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Western blot - Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832)All lanes : Biotin Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [FG4R] (ab173832) at 1/1000 dilution
Lane 1 : E. coli lysate containing a multi-epitope tagged protein at 0.25 µg
Lane 2 : E. coli lysate containing a multi-epitope tagged protein at 0.125 µg
Lane 3 : E. coli lysate containing a multi-epitope tagged protein at 0.0599999986588955 µg
Lane 4 : E. coli lysate containing a multi-epitope tagged protein at 0.0299999993294477 µg
Lane 5 : E. coli lysate containing a multi-epitope tagged protein at 0.0149999996647239 µg
Secondary
All lanes : Streptavidin-HRP at 1/20000 dilution
Developed using the ECL technique.Note: Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Incubation with ab173832 was for 1 hour at room temperature. Incubation with secondary antibody was for 30 miutes at room temperature.
Protocols
Datasheets and documents
References (0)
ab173832 has not yet been referenced specifically in any publications.
