Biotin Anti-DR5 antibody [MD5-1] (ab95618)
Key features and details
- Biotin Armenian hamster monoclonal [MD5-1] to DR5
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-DR5 antibody [MD5-1]
See all DR5 primary antibodies -
Description
Biotin Armenian hamster monoclonal [MD5-1] to DR5 -
Host species
Armenian hamster -
Conjugation
Biotin -
Tested Applications & Species
Application Species Flow Cyt Mouse -
Immunogen
Mouse DR5.
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Positive control
- Mouse DR5 transfected BHK cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MD5-1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Mouse
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Application | Abreviews | Notes |
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Flow Cyt |
Use 0.06-0.125µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.
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Notes |
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Flow Cyt
Use 0.06-0.125µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended. |
Target
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Function
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. -
Tissue specificity
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. -
Involvement in disease
Squamous cell carcinoma of the head and neck -
Sequence similarities
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 21933 Mouse
- SwissProt: Q9QZM4 Mouse
- Unigene: 193430 Mouse
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Alternative names
- Fas like protein antibody
- Apoptosis inducing protein TRICK2A/2B antibody
- Apoptosis inducing receptor TRAIL R2 antibody
see all
Images
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Flow cytometry staining of non-transfected (left) and mouse DR5 transfected (right) BHK cells with 0.06 µg of Golden Syrian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 µg ab95618 (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab95618 has not yet been referenced specifically in any publications.