Biotin Anti-DR5 antibody [MD5-1] (ab95618)

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Flow Cytometry - Biotin Anti-DR5 antibody [MD5-1] (ab95618)

    Key features and details

    • Biotin Armenian hamster monoclonal [MD5-1] to DR5
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Conjugation: Biotin
    • Isotype: IgG

    Overview

    • Product name

      Biotin Anti-DR5 antibody [MD5-1]
      See all DR5 primary antibodies

    • Description

      Biotin Armenian hamster monoclonal [MD5-1] to DR5

    • Host species

      Armenian hamster

    • Conjugation

      Biotin

    • Tested Applications & Species

      Application Species
      Flow Cyt
      Mouse
      See all applications and species data

    • Immunogen

      Mouse DR5.

    • Positive control

      • Mouse DR5 transfected BHK cells.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab95618 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Mouse
    Application Abreviews Notes
    Flow Cyt
    Use 0.06-0.125µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.
    Notes
    Flow Cyt
    Use 0.06-0.125µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended.

    Target

    • Function

      Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.

    • Tissue specificity

      Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.

    • Involvement in disease

      Squamous cell carcinoma of the head and neck

    • Sequence similarities

      Contains 1 death domain.
      Contains 3 TNFR-Cys repeats.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Fas like protein antibody
        • Apoptosis inducing protein TRICK2A/2B antibody
        • Apoptosis inducing receptor TRAIL R2 antibody
        • CD262 antibody
        • CD262 antigen antibody
        • Cytotoxic TRAIL receptor 2 antibody
        • Death domain containing receptor for TRAIL/Apo 2L antibody
        • Death receptor 5 antibody
        • DR5 antibody
        • KILLER antibody
        • KILLER/DR5 antibody
        • OTTHUMP00000123492 antibody
        • OTTHUMP00000123493 antibody
        • p53 regulated DNA damage inducible cell death receptor(killer) antibody
        • TNF related apoptosis inducing ligand receptor 2 antibody
        • TNF-related apoptosis-inducing ligand receptor 2 antibody
        • TNFRSF10B antibody
        • TR10B_HUMAN antibody
        • TRAIL R2 antibody
        • TRAIL receptor 2 antibody
        • TRAIL-R2 antibody
        • TRAILR2 antibody
        • TRICK2 antibody
        • TRICK2A antibody
        • TRICK2B antibody
        • TRICKB antibody
        • Tumor necrosis factor receptor like protein ZTNFR9 antibody
        • Tumor necrosis factor receptor superfamily member 10B antibody
        • Tumor necrosis factor receptor superfamily, member 10b antibody
        • ZTNFR9 antibody
        see all

      Images

      • Flow Cytometry - Biotin Anti-DR5 antibody [MD5-1] (ab95618)
        Flow Cytometry - Biotin Anti-DR5 antibody [MD5-1] (ab95618)
        Flow cytometry staining of non-transfected (left) and mouse DR5 transfected (right) BHK cells with 0.06 µg of Golden Syrian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 µg ab95618 (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.

      References (0)

      Publishing research using ab95618? Please let us know so that we can cite the reference in this datasheet.

      ab95618 has not yet been referenced specifically in any publications.

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