Biotin Anti-DR5 antibody [MD5-1] (ab95618)
![Flow Cytometry - Biotin Anti-DR5 antibody [MD5-1] (ab95618)](https://www.abcam.com/ps/products/95/ab95618/Images/DR5-Primary-antibodies-ab95618-3.jpg "Flow Cytometry - Biotin Anti-DR5 antibody [MD5-1] (ab95618)")
Key features and details
- Biotin Armenian hamster monoclonal [MD5-1] to DR5
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-DR5 antibody [MD5-1]
See all DR5 primary antibodies -
Description
Biotin Armenian hamster monoclonal [MD5-1] to DR5 -
Host species
Armenian hamster -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Mouse DR5.
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Positive control
- Mouse DR5 transfected BHK cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MD5-1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab95618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.06-0.125µg for 105-8 cells. Staining the cell sample in a final volume of 100 µl is recommended. |
Target
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Function
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. -
Tissue specificity
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. -
Involvement in disease
Squamous cell carcinoma of the head and neck -
Sequence similarities
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 21933 Mouse
- SwissProt: Q9QZM4 Mouse
- Unigene: 193430 Mouse
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Alternative names
- Fas like protein antibody
- Apoptosis inducing protein TRICK2A/2B antibody
- Apoptosis inducing receptor TRAIL R2 antibody
see all
Images
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Flow Cytometry - Biotin Anti-DR5 antibody [MD5-1] (ab95618)Flow cytometry staining of non-transfected (left) and mouse DR5 transfected (right) BHK cells with 0.06 µg of Golden Syrian Hamster IgG Isotype Control Biotin (open histogram) or 0.06 µg ab95618 (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.
Datasheets and documents
References (0)
ab95618 has not yet been referenced specifically in any publications.
