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Biotin Anti-EpCAM antibody [VU-1D9] (ab79079)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-EpCAM antibody [VU-1D9] (ab79079)

    Key features and details

    • Biotin Mouse monoclonal [VU-1D9] to EpCAM
    • Suitable for: IHC-P
    • Reacts with: Human
    • Conjugation: Biotin
    • Isotype: IgG1

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    Overview

    • Product name

      Biotin Anti-EpCAM antibody [VU-1D9]
      See all EpCAM primary antibodies
    • Description

      Biotin Mouse monoclonal [VU-1D9] to EpCAM
    • Host species

      Mouse
    • Conjugation

      Biotin
    • Specificity

      We have data to indicate that this antibody may not cross react with Rat or ferret. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

      ab79079 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
      Does not react with: Rat, Ferret
    • Immunogen

      Tissue, cells or virus corresponding to Human EpCAM.
      Database link: P16422

    • Positive control

      • Human colon carcinoma tissue.
    • General notes

      This product was changed from ascites to tissue culture supernatant on 29th September 2017 and product received after this date will be from tissue culture supernatant.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.05% Sodium azide
      Constituents: 0.41% PBS, 0.05% BSA
    • Concentration information loading...
    • Purity

      Protein A/G purified
    • Clonality

      Monoclonal
    • Clone number

      VU-1D9
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Tags & Cell Markers
      • Cell Type Markers
      • Epi / Endo-thelial

    Associated products

    • Alternative Versions

      • FITC Anti-EpCAM antibody [VU-1D9] (ab112067)
      • PE Anti-EpCAM antibody [VU-1D9] (ab112068)
      • PerCP/Cy5.5® Anti-EpCAM antibody [VU-1D9] (ab157319)
      • Anti-EpCAM antibody [VU-1D9] (ab187372)
      • Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)
      • Alexa Fluor® 647 Anti-EpCAM antibody [VU-1D9] (ab239273)
      • APC Anti-EpCAM antibody [VU-1D9] (ab239288)
    • Isotype control

      • Biotin Mouse IgG1, Kappa Monoclonal [MOPC-21] - isotype control (ab18434)
    • Recombinant Protein

      • Recombinant human EpCAM protein (ab155637)

    Applications

    Our Abpromise guarantee covers the use of ab79079 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P Use a concentration of 1 - 2 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

    Incubate for 30 min at RT. Staining of formalin/paraffin tissues requires digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37°C.

    Target

    • Function

      May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
    • Tissue specificity

      Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
    • Involvement in disease

      Defects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
      Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM.
    • Sequence similarities

      Belongs to the EPCAM family.
      Contains 1 thyroglobulin type-1 domain.
    • Post-translational
      modifications

      Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
    • Cellular localization

      Lateral cell membrane. Cell junction > tight junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction.
    • Target information above from: UniProt accession P16422 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4072 Human
      • Omim: 185535 Human
      • SwissProt: P16422 Human
      • Unigene: 542050 Human
      • Alternative names

        • 17 1A antibody
        • 323/A3 antibody
        • Adenocarcinoma associated antigen antibody
        • Adenocarcinoma-associated antigen antibody
        • Antigen identified by monoclonal antibody AUA1 antibody
        • AUA1 antibody
        • CD326 antibody
        • CD326 antigen antibody
        • Cell surface glycoprotein Trop 1 antibody
        • Cell surface glycoprotein Trop 2 antibody
        • Cell surface glycoprotein Trop-1 antibody
        • CO 17A antibody
        • CO17 1A antibody
        • CO17A antibody
        • DIAR5 antibody
        • EGP 2 antibody
        • EGP antibody
        • EGP2 antibody
        • EGP314 antibody
        • EGP40 antibody
        • Ep CAM antibody
        • Ep-CAM antibody
        • EPCAM antibody
        • EPCAM_HUMAN antibody
        • EpCAM1 antibody
        • Epithelial cell adhesion molecule antibody
        • Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) antibody
        • Epithelial cell surface antigen antibody
        • Epithelial cellular adhesion molecule antibody
        • Epithelial glycoprotein 1 antibody
        • Epithelial glycoprotein 314 antibody
        • Epithelial glycoprotein antibody
        • ESA antibody
        • GA733 1 antibody
        • GA733 2 antibody
        • GA733-2 antibody
        • gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein antibody
        • gp4 antibody
        • hEGP 2 antibody
        • hEGP314 antibody
        • HNPCC8 antibody
        • Human epithelial glycoprotein 2 antibody
        • KS 1/4 antigen antibody
        • KS1/4 antibody
        • KSA antibody
        • Ly74 antibody
        • Lymphocyte antigen 74 antibody
        • M1S 1 antibody
        • M1S2 antibody
        • M4S1 antibody
        • Major gastrointestinal tumor associated protein GA733 2 antibody
        • Major gastrointestinal tumor-associated protein GA733-2 antibody
        • mEGP314 antibody
        • Membrane component chromosome 4 surface marker (35kD glycoprotein) antibody
        • Membrane component, chromosome 4, surface marker 1 antibody
        • Membrane component, chromosome 4, surface marker antibody
        • MIC18 antibody
        • MK 1 antibody
        • Protein 289A antibody
        • TACD1 antibody
        • TACSTD1 antibody
        • TROP1 antibody
        • Tumor associated calcium signal transducer 1 antibody
        • Tumor associated calcium signal transducer 2 precursor antibody
        • Tumor-associated calcium signal transducer 1 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-EpCAM antibody [VU-1D9] (ab79079)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-EpCAM antibody [VU-1D9] (ab79079)

        ab79079 at 1-2 µg/mL staining EpCAM in human colon carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (5)

      Publishing research using ab79079? Please let us know so that we can cite the reference in this datasheet.

      ab79079 has been referenced in 5 publications.

      • Zaborowski MP  et al. Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles. Cell Rep 27:255-268.e6 (2019). PubMed: 30943406
      • Lee S & Ahn HJ Anti-EpCAM-conjugated adeno-associated virus serotype 2 for systemic delivery of EGFR shRNA: Its retargeting and antitumor effects on OVCAR3 ovarian cancer in vivo. Acta Biomater 91:258-269 (2019). PubMed: 31026519
      • Brinkmann F  et al. A Versatile Microarray Platform for Capturing Rare Cells. Sci Rep 5:15342 (2015). PubMed: 26493176
      • Casavant BP  et al. Efficient sample preparation from complex biological samples using a sliding lid for immobilized droplet extractions. Anal Chem 86:6355-62 (2014). PubMed: 24927449
      • Berry SM  et al. Purification of cell subpopulations via immiscible filtration assisted by surface tension (IFAST). Biomed Microdevices 13:1033-42 (2011). PubMed: 21796389

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Customer kindly called to inquire about a biotin conjugated primary antibody and associated tissue slide (IHC-P) to use with their streptavidin conjugated reagent.

      Read More

      Abcam community

      Verified customer

      Asked on May 18 2012

      Answer

      Thank you for contacting Abcam regarding finding biotin labeled primary antibodies and control tissues.

      After review of our catalog, my first recommendation is anti-Collagen VI, ab119300. This product is guaranteed in IHC-P and the images on the datasheet look very good when stained at a concentration of1.25µg/ml.Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      https://www.abcam.com/Collagen-VI-antibody-Biotin-ab119300.html

      The positive control for this is human liver tissue. We offer these slides as ab4348:

      https://www.abcam.com/Liver-human-normal-tissue-slides-ab4348.html



      A second recommendation would be our anti-Actin, ab79667. InIHC-P this has been used at 1/50 for 30 min with LV’s Ultra Vision, times and concentration may have to be adjusted experimentally. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min

      https://www.abcam.com/Actin-antibody-ACTN05-Biotin-ab79667.html

      The appropriate positive control tissue for this is skeletal muscle.

      https://www.abcam.com/Skeletal-muscle-human-normal-tissue-slides-ab4366.html



      A third choice might be EpCAM, ab79079. This product also has a very nice image on the datasheet. Useat 1-2µg/ml staining EpCAM in human breast carcinoma by IHC-P tissue using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells.

      EpCAM:https://www.abcam.com/EpCAM-antibody-VU-1D9-Biotin-ab79079.html

      Breast Carcinoma Tissue Slides:https://www.abcam.com/Breast-tumor-human-lobular-carcinoma-tissue-slides-ab4698.html



      I hope that you find this information to be helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

      Read More

      Abcam Scientific Support

      Answered on May 18 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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