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  1. Link

    biotin-f480-antibody-bm8-ab15694.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Biotin Anti-F4/80 antibody [BM8] (ab15694)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (6)

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Key features and details

  • Biotin Rat monoclonal [BM8] to F4/80
  • Reacts with: Mouse
  • Conjugation: Biotin
  • Isotype: IgG2a

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Protein
Product image
Recombinant Human EMR1/ADGRE1 protein (ab131740)
Conjugation
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Streptavidin Alexa Fluor® 488 Monovalent Antibody Labeling Kit (ab272187)

View more associated products

Overview

  • Product name

    Biotin Anti-F4/80 antibody [BM8]
    See all F4/80 primary antibodies
  • Description

    Biotin Rat monoclonal [BM8] to F4/80
  • Host species

    Rat
  • Conjugation

    Biotin
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Unfortunately, this information is considered to be commercially sensitive

  • Epitope

    F4/80 antigen.
  • Positive control

    • Macrophages in spleen, lymph nodes, thymus, liver, skin. Langerhans cells in the skin. Kupffer cells in the liver.
  • General notes

    The antigen is a 125kD cell surface membrane bound protein (non-reducing conditions). It is sensitive to 2 mercaptoethanol.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    BM8
  • Isotype

    IgG2a
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Tags & Cell Markers
    • Cell Type Markers
    • Other Cell Types
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker

Associated products

  • Alternative Versions

    • Anti-F4/80 antibody [BM8] (ab16911)
    • PerCP Anti-F4/80 antibody [BM8] (ab237026)
    • FITC Anti-F4/80 antibody [BM8] (ab60343)
  • Isotype control

    • Biotin Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18445)
  • Recombinant Protein

    • Recombinant Human EMR1/ADGRE1 protein (ab131740)

Target

  • Function

    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • Tissue specificity

    Expression is restricted to eosinophils.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession Q14246 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 13733 Mouse
    • SwissProt: Q61549 Mouse
    • Unigene: 2254 Mouse
    • Alternative names

      • ADGRE1 antibody
      • Adhesion G protein coupled receptor E1 antibody
      • Adhesion G protein-coupled receptor E1 antibody
      • AGRE1_HUMAN antibody
      • Cell surface glycoprotein EMR1 antibody
      • Cell surface glycoprotein F4/80 antibody
      • DD7A5 7 antibody
      • Egf like module containing mucin like hormone receptor like 1 antibody
      • Egf like module containing mucin like hormone receptor like sequence 1 antibody
      • EGF like module receptor 1 antibody
      • EGF TM7 antibody
      • EGF-like module receptor 1 antibody
      • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
      • EGFTM7 antibody
      • EMR 1 antibody
      • EMR1 antibody
      • EMR1 hormone receptor antibody
      • Gpf480 antibody
      • Ly71 antibody
      • Lymphocyte antigen 71 antibody
      • TM7LN3 antibody
      see all

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (6)

    Publishing research using ab15694? Please let us know so that we can cite the reference in this datasheet.

    ab15694 has been referenced in 6 publications.

    • Chen H  et al. Mouse Gamma Herpesvirus MHV-68 Induces Severe Gastrointestinal (GI) Dilatation in Interferon Gamma Receptor-Deficient Mice (IFN?R-/-) That Is Blocked by Interleukin-10. Viruses 10:N/A (2018). PubMed: 30249047
    • Duan T  et al. Dietary restriction protects against diethylnitrosamine-induced hepatocellular tumorigenesis by restoring the disturbed gene expression profile. Sci Rep 7:43745 (2017). PubMed: 28262799
    • Malek Mohammadi M  et al. The transcription factor GATA4 promotes myocardial regeneration in neonatal mice. EMBO Mol Med 9:265-279 (2017). PubMed: 28053183
    • Gerlini A  et al. The role of host and microbial factors in the pathogenesis of pneumococcal bacteraemia arising from a single bacterial cell bottleneck. PLoS Pathog 10:e1004026 (2014). Flow Cyt ; Mouse . PubMed: 24651834
    • Gumucio J  et al. T lymphocytes are not required for the development of fatty degeneration after rotator cuff tear. Bone Joint Res 3:262-72 (2014). IHC-Fr ; Rat . PubMed: 25185444
    • Qi X  et al. Mitochondrial protein nitration primes neurodegeneration in experimental autoimmune encephalomyelitis. J Biol Chem 281:31950-62 (2006). PubMed: 16920708

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [BM8] (Biotin)

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Specification
    Liver
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 20°C
    Read More

    Mr. Brendan Toohey

    Verified customer

    Submitted Nov 05 2010

    Question

    Customer has reported no signal in IHC-paraffin sections. Human samples. When this antibody was tested in IHC-paraffin sections, what was the protocol that was used?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 21 2005

    Answer

    Thank you for your enquiry and patience. I regret to inform you that this antibody has not yet been tested for application in IHC-P (paraffin sections) and so I cannot provide you with the protocol that was used. I have removed this application from the online datasheet and do apologize for this. If you would like a refund or credit note, please let me know. This antibody is expected to work in IHC-P however, as the unconjugated version of this clone, BM8, is suitable for this application. The non-biotinylated antibody reacts on paraffin sections after digestion with proteinase K (Dako, ready to use) for 10 minutes at room temperature.

    Read More

    Abcam Scientific Support

    Answered on Sep 27 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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