Biotin Anti-F4/80 antibody [BM8] (ab15694)
![Flow Cytometry - Biotin Anti-F4/80 antibody [BM8] (ab15694)](https://www.abcam.com/ps/products/15/ab15694/Images/ab15694-464325-pe-cy7-anti-cd62l-antibody-mel-14-flow-cytometry-bone-marrow-mouse.jpg "Flow Cytometry - Biotin Anti-F4/80 antibody [BM8] (ab15694)")
Key features and details
- Biotin Rat monoclonal [BM8] to F4/80
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Biotin Anti-F4/80 antibody [BM8]
See all F4/80 primary antibodies -
Description
Biotin Rat monoclonal [BM8] to F4/80 -
Host species
Rat -
Conjugation
Biotin -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Unfortunately, this information is considered to be commercially sensitive
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Epitope
F4/80 antigen. -
General notes
The antigen is a 125kD cell surface membrane bound protein (non-reducing conditions). It is sensitive to 2 mercaptoethanol.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
BM8 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
Tissue specificity
Expression is restricted to eosinophils. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 13733 Mouse
- SwissProt: Q61549 Mouse
- Unigene: 2254 Mouse
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Alternative names
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
Images
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Flow Cytometry - Biotin Anti-F4/80 antibody [BM8] (ab15694)
Flow Cytometry analysis of C57Bl/6 Mouse bone marrow cells labeling APC Anti-Mouse CD11b and 0.125 µg Biotin Anti-F4/80 antibody (ab15694) (right panel) or 0.125 µg Biotin Rat IgG2b isotype control (left panel), followed by Streptavidin PE.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab15694 has been referenced in 6 publications.
- Chen H et al. Mouse Gamma Herpesvirus MHV-68 Induces Severe Gastrointestinal (GI) Dilatation in Interferon Gamma Receptor-Deficient Mice (IFN?R-/-) That Is Blocked by Interleukin-10. Viruses 10:N/A (2018). PubMed: 30249047
- Duan T et al. Dietary restriction protects against diethylnitrosamine-induced hepatocellular tumorigenesis by restoring the disturbed gene expression profile. Sci Rep 7:43745 (2017). PubMed: 28262799
- Malek Mohammadi M et al. The transcription factor GATA4 promotes myocardial regeneration in neonatal mice. EMBO Mol Med 9:265-279 (2017). PubMed: 28053183
- Gerlini A et al. The role of host and microbial factors in the pathogenesis of pneumococcal bacteraemia arising from a single bacterial cell bottleneck. PLoS Pathog 10:e1004026 (2014). Flow Cyt ; Mouse . PubMed: 24651834
- Gumucio J et al. T lymphocytes are not required for the development of fatty degeneration after rotator cuff tear. Bone Joint Res 3:262-72 (2014). IHC-Fr ; Rat . PubMed: 25185444
- Qi X et al. Mitochondrial protein nitration primes neurodegeneration in experimental autoimmune encephalomyelitis. J Biol Chem 281:31950-62 (2006). PubMed: 16920708