Biotin Anti-FGF1 antibody (ab83163)
Key features and details
- Biotin Rabbit polyclonal to FGF1
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-FGF1 antibody
See all FGF1 primary antibodies -
Description
Biotin Rabbit polyclonal to FGF1 -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Highly pure (>98%) recombinant human FGF acidic
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Positive control
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab83163 is sterile filtered. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab83163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human FGF acidic. |
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| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 17 kDa. The detection limit for recombinant human FGF acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Target
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Function
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. - Information by UniProt
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Database links
- Entrez Gene: 2246 Human
- Omim: 131220 Human
- SwissProt: P05230 Human
- Unigene: 483635 Human
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Alternative names
- Acidic fibroblast growth factor antibody
- aFGF antibody
- Beta endothelial cell growth factor antibody
see all
Images
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Western blot - Biotin Anti-FGF1 antibody (ab83163)
To detect human FGF1 by Western Blot analysis ab83163 can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human FGF1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Image shown is non-reducing conditions; Human recombinant FGF1 protein at 250 - 0.24 ng/ml.
Lane 1 is MW marker, lanes 2-12 human recombinant FGF1 protein.
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Sandwich ELISA - Biotin Anti-FGF1 antibody (ab83163)To detect human FGF1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of ab83163 is required. This biotinylated polyclonal antibody, in conjunction with an anti-FGF1 (ab9588) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant human FGF1.
Datasheets and documents
References (0)
ab83163 has not yet been referenced specifically in any publications.
