Biotin Anti-Fibronectin antibody (ab6584)
Key features and details
- Biotin Rabbit polyclonal to Fibronectin
- Suitable for: IHC-P, WB
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Fibronectin antibody
See all Fibronectin primary antibodies -
Description
Biotin Rabbit polyclonal to Fibronectin -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mammals -
Immunogen
Full length native protein (purified) corresponding to Human Fibronectin. Purified from human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration.
Database link: P02751 -
General notes
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. -
Primary antibody notes
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200.
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|
WB |
1/5000 - 1/10000.
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Notes |
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IHC-P
1/50 - 1/200. |
WB
1/5000 - 1/10000. |
Target
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Function
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. -
Tissue specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine. -
Involvement in disease
Glomerulopathy with fibronectin deposits 2 -
Sequence similarities
Contains 12 fibronectin type-I domains.
Contains 2 fibronectin type-II domains.
Contains 16 fibronectin type-III domains. -
Developmental stage
Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. -
Post-translational
modificationsSulfated.
It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
Phosphorylated by FAM20C in the extracellular medium.
Proteolytic processing produces the C-terminal NC1 peptide, anastellin. -
Cellular localization
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 2335 Human
- Omim: 135600 Human
- SwissProt: P02751 Human
- Unigene: 203717 Human
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Alternative names
- CIG antibody
- Cold insoluble globulin antibody
- Cold-insoluble globulin antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 6.0, labelling Fibronectin with ab6584 at a concentration of 10 µg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 9.0, labelling Fibronectin with ab6584 at a concentration of 10 µg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (41)
ab6584 has been referenced in 41 publications.
- Bonvallet PP et al. Biophysical Characterization of a Novel Tri-Layer Placental Allograft Membrane. Adv Wound Care (New Rochelle) N/A:N/A (2021). PubMed: 33975444
- Yemanyi F et al. Dexamethasone and Glucocorticoid-Induced Matrix Temporally Modulate Key Integrins, Caveolins, Contractility, and Stiffness in Human Trabecular Meshwork Cells. Invest Ophthalmol Vis Sci 61:16 (2020). PubMed: 33170205
- Osmond MJ et al. Human trabecular meshwork cell behavior is influenced by collagen scaffold pore architecture and glycosaminoglycan composition. Biotechnol Bioeng 117:3150-3159 (2020). PubMed: 32589791
- Moore MC et al. Biological characterization of dehydrated amniotic membrane allograft: Mechanisms of action and implications for wound care. J Biomed Mater Res B Appl Biomater N/A:N/A (2020). PubMed: 32458591
- Gay D et al. Phagocytosis of Wnt inhibitor SFRP4 by late wound macrophages drives chronic Wnt activity for fibrotic skin healing. Sci Adv 6:eaay3704 (2020). PubMed: 32219160