Biotin Anti-Flagellin antibody (ab193301)
Key features and details
- Biotin Rabbit polyclonal to Flagellin
- Suitable for: ELISA
- Reacts with: Escherichia coli
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Flagellin antibody
See all Flagellin primary antibodies -
Description
Biotin Rabbit polyclonal to Flagellin -
Host species
Rabbit -
Conjugation
Biotin -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Recombinant full length protein corresponding to Escherichia coli Flagellin aa 1 to the C-terminus.
Database link: P04949 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Store In the Dark. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Caprylic Acid - Ammonium Sulfate precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Flagellin (FliC) is a subunit protein that polymerizes (along with other proteins) to form the filaments of bacterial flagella. Assembly of the bacterial flagellum occurs in a precise, temporal order where the basal component (FlgE, FlgK, and FlgL are assembled inside the bacterial membrane, followed by exportation of the filament cap protein FliD, and secretion of about 20,000 flagellin monomers (FliC) through the channel. FliC monomers are polymerized to form the tail filament. FliC monomers can function as pathogen-associated molecular patterns (PAMPs), and can be detected by host cells through surface-localized toll-like receptor 5 (TLR5) and cytosolic Nod-like receptors (NLRs). -
Cellular localization
Secreted. Bacterial flagellum. -
Database links
- Entrez Gene: 949101 Escherichia coli
- Entrez Gene: 961921 Escherichia coli
- SwissProt: P04949 Escherichia coli
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Alternative names
- 41 kDa antigen antibody
- b1923 antibody
- flaF antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab193301 has been referenced in 1 publication.
- Braathen R et al. The Magnitude and IgG Subclass of Antibodies Elicited by Targeted DNA Vaccines Are Influenced by Specificity for APC Surface Molecules. Immunohorizons 2:38-53 (2018). PubMed: 31022690