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    biotin-fluorescein-antibody-ab6655.pdf

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Biotin Anti-Fluorescein antibody (ab6655)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (2)

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Western blot - Biotin Anti-Fluorescein antibody (ab6655)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fluorescein antibody (ab6655)

Key features and details

  • Biotin Goat polyclonal to Fluorescein
  • Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, In situ hybridization, WB
  • Reacts with: Species independent
  • Conjugation: Biotin
  • Isotype: IgG

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Conjugation
Product image
Streptavidin Alexa Fluor® 488 Monovalent Antibody Labeling Kit (ab272187)

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Overview

  • Product name

    Biotin Anti-Fluorescein antibody
    See all Fluorescein primary antibodies
  • Description

    Biotin Goat polyclonal to Fluorescein
  • Host species

    Goat
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, In situ hybridization, WBmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Fluorescein conjugated to Goat IgG

  • General notes

    Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Conjugates

Associated products

  • Isotype control

    • Biotin Goat IgG - Isotype Control (ab37376)

Applications

Our Abpromise guarantee covers the use of ab6655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.
IHC-P 1/1000 - 1/5000.
IHC-Fr 1/1000 - 1/5000.
Immunomicroscopy Use at an assay dependent concentration.
In situ hybridization Use at an assay dependent concentration.
WB 1/2000 - 1/10000.

Target

  • Relevance

    Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • Alternative names

    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

Images

  • Western blot - Biotin Anti-Fluorescein antibody (ab6655)
    Western blot - Biotin Anti-Fluorescein antibody (ab6655)
    Biotin Anti-Fluorescein antibody (ab6655) at 1/1000 dilution + BSA conjugated Fluorescein.

    Secondary
    Peroxidase conjugated Streptavidin secondary antibody at 1/40000 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fluorescein antibody (ab6655)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fluorescein antibody (ab6655)Cropped image from Long et al, Nature Communications, 7, 10354, Fig. 7a.; doi: 10.1038/ncomms10354 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistochemical analysis of chicken embryo neuroepithelium labelling fluorescein-tagged morpholinos with ab6655. Samples were fixed in 4% paraformaldehyde at 4°C for 1 hour.

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab6655? Please let us know so that we can cite the reference in this datasheet.

    ab6655 has been referenced in 2 publications.

    • He B  et al. Vascular targeting of LIGHT normalizes blood vessels in primary brain cancer and induces intratumoural high endothelial venules. J Pathol 245:209-221 (2018). PubMed: 29603739
    • Long K  et al. Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin. Nat Commun 7:10354 (2016). IHC-Fr, IHC-FrFl . PubMed: 26838601

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Thank you for your information. This inquiry is about #ab6655. Please let me know. Thanks. We received an inquiry about the reactivity of anti FlTC from our customer. They use Fluorescein Sodium and they would like to detect Fluorescein Sodium by using your anti-FITC. Can this product recognized Fluorescein Sodium by using this antibody ?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 01 2004

    Answer

    Thank you for your enquiry. I'm sorry but we don't have any information about this antibody being able to detect Fluorescein Sodium. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Nov 02 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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