Key features and details
- Biotin Mouse monoclonal [BT.6A4] to Fluorescein
- Suitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgM
Product nameBiotin Anti-Fluorescein antibody [BT.6A4]
See all Fluorescein primary antibodies
DescriptionBiotin Mouse monoclonal [BT.6A4] to Fluorescein
SpecificityThe antibody reacts with free and bound fluorescein.
Tested applicationsSuitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridizationmore details
Species reactivityReacts with: Species independent
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purification notesPurified anti-fluorescein biotin conjugate.
Light chain typekappa
Our Abpromise guarantee covers the use of ab6215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|ELISA||1/1000 - 1/10000.|
|IHC-P||1/200 - 1/2000.|
|IHC-Fr||1/200 - 1/2000.|
|In situ hybridization||Use at an assay dependent dilution.|
RelevanceFluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
ab6215 has been referenced in 1 publication.
- Mukaiyama M et al. Non-electrophilic TRPA1 agonists, menthol, carvacrol and clotrimazole, open epithelial tight junctions via TRPA1 activation. J Biochem N/A:N/A (2020). PubMed: 32428205